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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_07355 and AO356_07360 overlap by 4 nucleotides
AO356_07360 and AO356_07365 are separated by 64 nucleotides
AO356_07365 and AO356_07370 are separated by 223 nucleotides
AO356_07370 and AO356_07375 are separated by 38 nucleotides
AO356_07355: AO356_07355 - hypothetical protein, at 1,630,740 to 1,631,576
_07355
AO356_07360: AO356_07360 - N-acetyl-anhydromuranmyl-L-alanine amidase, at 1,631,573 to 1,632,133
_07360
AO356_07365: AO356_07365 - thymidine phosphorylase, at 1,632,198 to 1,634,411
_07365
AO356_07370: AO356_07370 - nicotinate-nucleotide pyrophosphorylase, at 1,634,635 to 1,635,483
_07370
AO356_07375: AO356_07375 - dephospho-CoA kinase, at 1,635,522 to 1,635,827
_07375
Group
Condition
AO356
_07355
AO356
_07360
AO356
_07365
AO356
_07370
AO356
_07375
pH
Minimal media pH9
+0.2
-0.6
-0.1
-5.2
-0.2
carbon source
NAG (C)
+0.1
-0.1
+0.1
-4.2
-0.2
pH
Minimal media pH8
-0.0
-0.3
-0.0
-3.6
-0.1
pH
Minimal media pH8
+0.1
-0.4
-0.0
-2.9
-0.3
survival
4C survival; 192 hrs
-0.3
-1.0
-0.1
-0.8
-0.6
carbon source
Carbon source D-Mannitol 10 mM
-0.5
+0.0
+0.0
-1.1
-0.8
carbon source
L-Arginine (C)
-0.1
-0.0
-0.1
-1.5
-0.4
motility
outer cut, LB soft agar motility assay
+0.4
-2.2
-0.9
+0.5
+0.2
pH
Minimal media pH7
+0.0
-0.0
+0.0
-1.5
-0.5
nitrogen source
D-Serine (N)
-0.2
-2.4
+0.1
-0.1
+0.6
motility
outer cut, LB soft agar motility assay
+0.2
-1.7
-0.5
-0.0
+0.1
nitrogen source
nitrite (N)
+0.2
-0.2
-0.1
-2.2
+0.4
survival
4C survival; 672 hrs
-0.5
-0.9
+0.1
-0.4
-0.0
nitrogen source
Ammonium chloride (N)
+0.1
+0.2
-0.1
-2.2
+0.2
carbon source
L-Glutamic (C)
-0.0
-0.2
-0.1
-1.6
+0.1
survival
4C survival; 1152 hrs
-0.6
-1.0
+0.2
-0.2
-0.1
carbon source
acetate (C)
-0.2
-0.8
+0.1
-0.9
+0.4
motility
outer cut, LB soft agar motility assay
-0.6
-0.7
-0.6
+0.5
+0.0
pH
Minimal media pH7
+0.1
-0.1
+0.0
-1.5
+0.2
phage
P5ss-5 phage 10-1 dilution SM calcl2
+0.3
-1.3
+0.1
+0.2
-0.5
survival
4C survival; 360 hrs
+0.2
-0.9
+0.0
+0.3
-0.7
carbon source
L-Glutamic (C)
+0.3
+0.2
+0.0
-1.2
-0.3
nitrogen source
Adenosine (N)
+0.1
+0.3
-0.1
-1.3
+0.2
stress
acetate 40 mM
+0.2
-0.4
+0.6
-0.8
-0.1
motility
outer cut, LB soft agar motility assay
+0.4
-1.2
-0.4
+0.8
-0.1
nitrogen source
Putrescine (N)
+0.0
+0.3
+0.1
-1.2
+0.2
stress
copper (II) chloride 2.5 mM
+0.2
-0.4
+0.3
-0.8
+0.8
carbon source
D-Xylose (C)
-0.2
+0.3
+0.0
+1.0
+0.5
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
+0.5
-0.0
+0.0
+0.7
+0.9
carbon source
Inosine (C)
+0.1
+0.3
+0.1
+1.7
+0.4
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