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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_07315 and AO356_07320 are separated by 134 nucleotides
AO356_07320 and AO356_07325 are separated by 201 nucleotides
AO356_07325 and AO356_07330 are separated by 14 nucleotides
AO356_07330 and AO356_07335 overlap by 1 nucleotides
AO356_07315: AO356_07315 - flavodoxin, at 1,616,226 to 1,618,769
_07315
AO356_07320: AO356_07320 - alkaline phosphatase, at 1,618,904 to 1,620,445
_07320
AO356_07325: AO356_07325 - fructose phosphotransferase system, IIB/IIC components (EC 2.7.1.202)
(from data)
, at 1,620,647 to 1,622,389
_07325
AO356_07330: AO356_07330 - 1-phosphofructokinase (EC 2.7.1.56)
(from data)
, at 1,622,404 to 1,623,345
_07330
AO356_07335: AO356_07335 - fructose-specific PTS system (fructose 1-phosphate forming), EI, Hpr, and EII-A components
(from data)
, at 1,623,345 to 1,626,209
_07335
Group
Condition
AO356
_07315
AO356
_07320
AO356
_07325
AO356
_07330
AO356
_07335
carbon source
D-Fructose (C)
-0.1
+0.1
-2.8
-3.3
-2.5
carbon source
D-Fructose (C)
+0.2
-0.1
-2.4
-2.8
-2.6
pH
Minimal media pH9
+0.0
+0.1
-1.3
-1.2
-1.6
carbon source
D-Mannose (C)
+0.0
+0.2
-0.8
-0.6
-0.7
motility
outer cut, LB soft agar motility assay
+0.1
-0.2
+0.2
-0.7
-0.8
phage
P5ss-5 phage 10-1 dilution SM calcl2
-0.0
-0.3
-0.4
-0.4
-0.1
carbon source
L-Histidine (C)
+0.1
+0.0
-0.1
-1.0
-0.1
carbon source
L-Histidine (C)
+0.1
-0.0
-0.2
-0.6
-0.3
stress
Nickel (II) chloride 0.625 mM
+0.0
-0.0
-0.5
-0.2
-0.3
carbon source
D-Glucosamine Hydrochloride (C)
-0.1
+0.1
-0.4
-0.1
-0.3
stress
Nickel (II) chloride 0.625 mM
+0.2
-0.0
-0.2
-0.4
-0.5
carbon source
L-Lactate (C)
+0.1
-0.1
-0.3
-0.2
-0.3
motility
outer cut, LB soft agar motility assay
-0.2
-0.3
+0.1
-0.3
-0.1
carbon source
D-Xylose (C)
-0.0
+0.2
-0.0
-0.4
-0.5
motility
outer cut, LB soft agar motility assay
-0.4
+0.2
+0.0
-0.3
-0.2
stress
Rifampicin 0.0024 mg/ml
+0.5
+0.0
-0.4
-0.3
-0.5
carbon source
D-Galactose (C)
+0.1
+0.0
-0.2
-0.4
-0.3
motility
outer cut, LB soft agar motility assay
-0.3
-0.1
+0.2
-0.6
+0.2
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
+0.1
-0.5
-0.2
-0.4
+0.6
stress
Benzalkonium Chloride 0.008 mg/ml
+0.4
+0.1
-0.3
-0.1
-0.4
stress
nitrate 200 mM
+0.3
+0.1
-0.2
-0.3
-0.2
stress
Benzalkonium Chloride 0.016 mg/ml
+0.4
+0.0
-0.3
-0.1
-0.2
stress
Sisomicin 0.001 mg/ml
-0.1
+0.1
-0.3
-0.3
+0.4
carbon source
D-Gluconic Acid (C)
+0.2
-0.1
-0.2
+0.5
-0.3
anaerobic
Nitrate reduction with yeast extract
-0.1
-0.4
+0.2
+0.3
+0.3
carbon source
Glycerol (C)
+0.1
-0.1
-0.1
+0.6
+0.2
carbon source
Glycerol (C)
+0.1
+0.0
+0.4
+0.6
+0.1
stress
nitrite 10 mM
-0.0
+0.3
+0.4
+0.4
+0.2
stress
KB-half, Pseudo5 supernatant
+0.1
-0.1
-0.0
+0.6
+0.7
carbon source
Inosine (C)
-0.0
+0.0
+0.2
+1.3
+0.3
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