Fitness for 5 genes in Pseudomonas fluorescens FW300-N2C3

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500 ntAO356_06600 and AO356_06605 are separated by 12 nucleotidesAO356_06605 and AO356_06610 are separated by 28 nucleotidesAO356_06610 and AO356_06615 are separated by 88 nucleotidesAO356_06615 and AO356_06620 are separated by 13 nucleotides AO356_06600: AO356_06600 - pilus assembly protein PilW, at 1,462,184 to 1,462,942 _06600 AO356_06605: AO356_06605 - 23S rRNA (adenine(2503)-C2)-methyltransferase, at 1,462,955 to 1,464,103 _06605 AO356_06610: AO356_06610 - nucleoside diphosphate kinase, at 1,464,132 to 1,464,557 _06610 AO356_06615: AO356_06615 - FeS assembly protein IscX, at 1,464,646 to 1,464,846 _06615 AO356_06620: AO356_06620 - 2Fe-2S ferredoxin, at 1,464,860 to 1,465,201 _06620
Group Condition AO356_06600 AO356_06605 AO356_06610 AO356_06615 AO356_06620
carbon source D-Lactate (C) -0.1 -0.2 N.D. -4.0 N.D.
carbon source L-Arabinose (C) +0.0 -0.8 N.D. -2.6 N.D.
phage P5ss-5 phage 10-1 dilution SM calcl2 -0.4 -0.1 N.D. -2.7 N.D.
carbon source L-Arabinose (C) +0.1 -1.0 N.D. -1.5 N.D.
lb LB -0.0 -0.2 N.D. -2.1 N.D.
carbon source D,L-Lactate (C) -0.2 -0.3 N.D. -1.7 N.D.
motility outer cut, LB soft agar motility assay +0.3 -0.6 N.D. -1.8 N.D.
resistance 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine -1.0 -0.2 N.D. -0.9 N.D.
carbon source L-Lactate (C) -0.1 -0.1 N.D. -1.8 N.D.
motility outer cut, LB soft agar motility assay +0.3 -2.3 N.D. +0.2 N.D.
carbon source D-Glucose (C) +0.1 -1.2 N.D. -0.5 N.D.
stress Spectinomycin 0.0125 mg/ml -0.2 +0.0 N.D. -1.5 N.D.
carbon source L-Asparagine (C) +0.1 -0.5 N.D. -1.2 N.D.
carbon source D-Lactate (C) -0.3 +0.4 N.D. -1.6 N.D.
anaerobic Nitrate reduction with yeast extract +0.5 -0.7 N.D. -1.3 N.D.
stress methylglyoxal 0.016 vol% -0.7 +1.3 N.D. -1.9 N.D.
nitrogen source D-Glucosamine Hydrochloride (N) +0.2 +0.1 N.D. -1.5 N.D.
stress Rifampicin 0.0016 mg/ml +0.2 +0.2 N.D. -1.4 N.D.
stress Tetracycline 0.0001 mg/ml -0.1 +0.6 N.D. -1.2 N.D.
carbon source Carbon source Sucrose 10 mM +0.0 -1.1 N.D. +0.6 N.D.
stress Benzalkonium Chloride 0.016 mg/ml +0.3 +0.9 N.D. -1.5 N.D.
stress perchlorate 75 mM +0.9 -0.6 N.D. -0.4 N.D.
survival 4C survival; 24 hrs -0.2 -0.8 N.D. +0.9 N.D.
stress Thallium(I) acetate 0.008 mg/ml +0.9 -0.9 N.D. +0.0 N.D.
stress Cholin acetate 40 mM +0.2 -0.7 N.D. +0.8 N.D.
nitrogen source D-Serine (N) -0.7 +1.1 N.D. +0.1 N.D.
stress Thallium(I) acetate 0.008 mg/ml +0.9 -0.9 N.D. +0.8 N.D.
motility outer cut, LB soft agar motility assay +0.0 -0.3 N.D. +1.4 N.D.
motility outer cut, LB soft agar motility assay +0.3 -0.8 N.D. +1.7 N.D.
stress copper (II) chloride 2.5 mM +1.2 +0.1 N.D. +1.1 N.D.
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