Fitness for 5 genes in Pseudomonas fluorescens FW300-N2C3

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500 ntAO356_06305 and AO356_06310 overlap by 11 nucleotidesAO356_06310 and AO356_06315 are separated by 156 nucleotidesAO356_06315 and AO356_06320 are separated by 146 nucleotidesAO356_06320 and AO356_06325 overlap by 38 nucleotides AO356_06305: AO356_06305 - spermidine/putrescine ABC transporter permease, at 1,397,916 to 1,398,863 _06305 AO356_06310: AO356_06310 - spermidine/putrescine ABC transporter permease, at 1,398,853 to 1,399,671 _06310 AO356_06315: AO356_06315 - 1-pyrroline dehydrogenase, at 1,399,828 to 1,401,252 _06315 AO356_06320: AO356_06320 - transcriptional regulator, at 1,401,399 to 1,401,662 _06320 AO356_06325: AO356_06325 - plasmid stabilization protein ParE, at 1,401,625 to 1,401,924 _06325
Group Condition AO356_06305 AO356_06310 AO356_06315 AO356_06320 AO356_06325
nitrogen source D-Alanine (N) +0.1 -0.2 -0.1 +0.1 -3.1
pH Minimal media pH6 +0.0 -0.3 -0.1 +0.1 -1.7
stress Benzalkonium Chloride 0.008 mg/ml -0.0 -0.6 +0.1 -0.5 -0.9
nitrogen source Thymine (N) -0.1 -0.2 -0.2 -0.5 -1.0
carbon source Carbon source D-Mannitol 10 mM -0.0 -0.1 -0.2 +0.3 -1.8
temperature LB 10C +0.1 -0.1 -0.0 -0.2 -1.6
carbon source pyruvate (C) +0.1 -0.3 +0.1 -0.3 -1.2
carbon source Carbon source Sucrose 5 mM +0.1 -0.2 -0.0 +0.5 -2.2
anaerobic Nitrate reduction with yeast extract -0.2 -0.2 -0.1 -1.4 +0.2
carbon source D-Xylose (C) +0.1 -0.0 -0.1 -0.3 -1.2
nitrogen source Putrescine (N) -0.1 -0.2 +0.2 -0.2 -1.3
stress nitrite 10 mM -0.1 -0.2 +0.1 -0.3 -0.9
stress Spectinomycin 0.0125 mg/ml +0.1 -0.1 -0.1 +0.1 -1.2
stress Sisomicin 0.001 mg/ml -0.3 -0.5 +0.3 -0.7 -0.1
stress nitrate 200 mM +0.1 -0.2 -0.0 +0.6 -1.7
carbon source Carbon source D-Mannitol 10 mM +0.1 -0.4 +0.0 -0.4 -0.6
carbon source L-Lysine (C) -0.0 +0.4 +0.0 +0.4 -2.0
survival 4C survival; 360 hrs -0.1 +0.0 +0.0 +0.2 -1.2
carbon source Carbon source Sucrose 10 mM +0.1 -0.5 -0.0 +0.4 -0.8
carbon source L-Lysine (C) -0.0 +0.6 -0.2 -0.6 -0.6
temperature LB 30C +0.0 -0.1 +0.2 +0.5 -1.2
motility outer cut, LB soft agar motility assay -0.2 -0.4 +0.3 -0.6 +0.3
stress Rifampicin 0.0016 mg/ml +0.1 +0.4 -0.1 +0.6 -1.5
carbon source L-Malic (C) +0.1 +0.7 -0.0 -0.3 -0.7
stress KB-half, Pseudo5 supernatant +0.2 +0.8 +0.2 +0.1 -1.3
stress Choline chloride 500 mM -0.1 -0.4 -0.2 -0.0 +0.9
motility outer cut, LB soft agar motility assay -0.4 -0.0 -0.3 +0.0 +1.0
carbon source L-Lactate (C) -0.0 -0.1 +0.2 -0.5 +0.7
carbon source D-Fructose (C) -0.1 +0.0 +0.1 -0.4 +1.4
stress KB-half, Pseudo11 supernatant, 100 uM FeCl3 +0.1 +0.9 +0.4 -0.1 -0.1
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