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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_06305 and AO356_06310 overlap by 11 nucleotides
AO356_06310 and AO356_06315 are separated by 156 nucleotides
AO356_06315 and AO356_06320 are separated by 146 nucleotides
AO356_06320 and AO356_06325 overlap by 38 nucleotides
AO356_06305: AO356_06305 - spermidine/putrescine ABC transporter permease, at 1,397,916 to 1,398,863
_06305
AO356_06310: AO356_06310 - spermidine/putrescine ABC transporter permease, at 1,398,853 to 1,399,671
_06310
AO356_06315: AO356_06315 - 1-pyrroline dehydrogenase, at 1,399,828 to 1,401,252
_06315
AO356_06320: AO356_06320 - transcriptional regulator, at 1,401,399 to 1,401,662
_06320
AO356_06325: AO356_06325 - plasmid stabilization protein ParE, at 1,401,625 to 1,401,924
_06325
Group
Condition
AO356
_06305
AO356
_06310
AO356
_06315
AO356
_06320
AO356
_06325
nitrogen source
D-Alanine (N)
+0.1
-0.2
-0.1
+0.1
-3.1
pH
Minimal media pH6
+0.0
-0.3
-0.1
+0.1
-1.7
stress
Benzalkonium Chloride 0.008 mg/ml
-0.0
-0.6
+0.1
-0.5
-0.9
nitrogen source
Thymine (N)
-0.1
-0.2
-0.2
-0.5
-1.0
carbon source
Carbon source D-Mannitol 10 mM
-0.0
-0.1
-0.2
+0.3
-1.8
temperature
LB 10C
+0.1
-0.1
-0.0
-0.2
-1.6
carbon source
pyruvate (C)
+0.1
-0.3
+0.1
-0.3
-1.2
carbon source
Carbon source Sucrose 5 mM
+0.1
-0.2
-0.0
+0.5
-2.2
anaerobic
Nitrate reduction with yeast extract
-0.2
-0.2
-0.1
-1.4
+0.2
carbon source
D-Xylose (C)
+0.1
-0.0
-0.1
-0.3
-1.2
nitrogen source
Putrescine (N)
-0.1
-0.2
+0.2
-0.2
-1.3
stress
nitrite 10 mM
-0.1
-0.2
+0.1
-0.3
-0.9
stress
Spectinomycin 0.0125 mg/ml
+0.1
-0.1
-0.1
+0.1
-1.2
stress
Sisomicin 0.001 mg/ml
-0.3
-0.5
+0.3
-0.7
-0.1
stress
nitrate 200 mM
+0.1
-0.2
-0.0
+0.6
-1.7
carbon source
Carbon source D-Mannitol 10 mM
+0.1
-0.4
+0.0
-0.4
-0.6
carbon source
L-Lysine (C)
-0.0
+0.4
+0.0
+0.4
-2.0
survival
4C survival; 360 hrs
-0.1
+0.0
+0.0
+0.2
-1.2
carbon source
Carbon source Sucrose 10 mM
+0.1
-0.5
-0.0
+0.4
-0.8
carbon source
L-Lysine (C)
-0.0
+0.6
-0.2
-0.6
-0.6
temperature
LB 30C
+0.0
-0.1
+0.2
+0.5
-1.2
motility
outer cut, LB soft agar motility assay
-0.2
-0.4
+0.3
-0.6
+0.3
stress
Rifampicin 0.0016 mg/ml
+0.1
+0.4
-0.1
+0.6
-1.5
carbon source
L-Malic (C)
+0.1
+0.7
-0.0
-0.3
-0.7
stress
KB-half, Pseudo5 supernatant
+0.2
+0.8
+0.2
+0.1
-1.3
stress
Choline chloride 500 mM
-0.1
-0.4
-0.2
-0.0
+0.9
motility
outer cut, LB soft agar motility assay
-0.4
-0.0
-0.3
+0.0
+1.0
carbon source
L-Lactate (C)
-0.0
-0.1
+0.2
-0.5
+0.7
carbon source
D-Fructose (C)
-0.1
+0.0
+0.1
-0.4
+1.4
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
+0.1
+0.9
+0.4
-0.1
-0.1
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