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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_06270 and AO356_06275 overlap by 1 nucleotides
AO356_06275 and AO356_06280 are separated by 223 nucleotides
AO356_06280 and AO356_06285 are separated by 184 nucleotides
AO356_06285 and AO356_06290 are separated by 215 nucleotides
AO356_06270: AO356_06270 - chemotaxis protein CheW, at 1,389,594 to 1,390,130
_06270
AO356_06275: AO356_06275 - chemotaxis protein, at 1,390,130 to 1,391,752
_06275
AO356_06280: AO356_06280 - hypothetical protein, at 1,391,976 to 1,392,686
_06280
AO356_06285: AO356_06285 - LysR family transcriptional regulator, at 1,392,871 to 1,393,785
_06285
AO356_06290: AO356_06290 - 1-pyrroline dehydrogenase, at 1,394,001 to 1,395,458
_06290
Group
Condition
AO356
_06270
AO356
_06275
AO356
_06280
AO356
_06285
AO356
_06290
stress
copper (II) chloride 2.5 mM
N.D.
+0.0
-0.4
-0.6
+0.0
anaerobic
Nitrate reduction with yeast extract
N.D.
-0.3
-0.9
+0.1
+0.1
survival
4C survival; 360 hrs
N.D.
+0.1
-0.5
-0.3
-0.1
carbon source
Ethanol (C)
N.D.
-0.0
-0.3
-0.3
-0.1
stress
perchlorate 75 mM
N.D.
+0.2
-0.3
-0.3
-0.2
carbon source
D-Alanine (C)
N.D.
+0.1
-0.1
-0.4
-0.1
stress
Cholin acetate 20 mM
N.D.
+0.1
-0.5
-0.2
-0.0
temperature
LB 30C
N.D.
+0.1
-0.4
-0.2
-0.0
stress
1-ethyl-3-methylimidazolium chloride 200 mM
N.D.
+0.3
-0.1
-0.5
-0.1
stress
Sisomicin 0.001 mg/ml
N.D.
+0.1
+0.1
-0.2
-0.4
phage
P5ss-5 phage 10-1 dilution SM calcl2
N.D.
+0.2
-0.5
+0.0
-0.2
carbon source
NAG (C)
N.D.
+0.0
-0.6
+0.3
+0.0
stress
Choline chloride 300 mM
N.D.
+0.3
-0.3
-0.1
-0.1
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
N.D.
-0.1
+0.3
-0.7
+0.2
stress
1-ethyl-3-methylimidazolium acetate 10 mM
N.D.
+0.1
+0.2
-0.3
-0.2
motility
outer cut, LB soft agar motility assay
N.D.
+0.1
-0.9
+0.6
+0.1
stress
Benzalkonium Chloride 0.016 mg/ml
N.D.
-0.0
+0.4
-0.4
+0.0
stress
Lomefloxacin 0.0005 mM
N.D.
-0.2
-0.2
+0.3
+0.0
stress
Benzethonium chloride 0.025 mM
N.D.
-0.0
+0.4
-0.4
-0.0
stress
KB-half, Pseudo5 supernatant
N.D.
+0.0
+0.4
-0.7
+0.4
carbon source
L-Arabinose (C)
N.D.
+0.2
+0.3
-0.3
+0.0
stress
nitrite 10 mM
N.D.
+0.2
+0.1
+0.3
-0.2
stress
Fusidic 0.1 mg/ml
N.D.
+0.2
+0.5
-0.1
-0.1
carbon source
D-Glucose (C)
N.D.
+0.1
+0.4
-0.1
+0.1
stress
Spectinomycin 0.025 mg/ml
N.D.
+0.1
+0.3
+0.3
-0.1
stress
nitrate 200 mM
N.D.
+0.3
-0.1
+0.4
+0.1
carbon source
a-Ketoglutaric (C)
N.D.
+0.3
+0.5
-0.2
+0.0
carbon source
D-Lactate (C)
N.D.
-0.0
+0.4
+0.1
+0.2
carbon source
D-Fructose (C)
N.D.
+0.2
+0.6
-0.2
+0.1
motility
outer cut, LB soft agar motility assay
N.D.
+0.1
+0.4
+0.2
+0.1
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