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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_06215 and AO356_06220 are separated by 148 nucleotides
AO356_06220 and AO356_06225 are separated by 36 nucleotides
AO356_06225 and AO356_06230 are separated by 151 nucleotides
AO356_06230 and AO356_06235 are separated by 101 nucleotides
AO356_06215: AO356_06215 - alpha/beta hydrolase, at 1,376,492 to 1,377,397
_06215
AO356_06220: AO356_06220 - hypothetical protein, at 1,377,546 to 1,378,823
_06220
AO356_06225: AO356_06225 - hypothetical protein, at 1,378,860 to 1,379,414
_06225
AO356_06230: AO356_06230 - TetR family transcriptional regulator, at 1,379,566 to 1,380,282
_06230
AO356_06235: AO356_06235 - lysine--tRNA ligase, at 1,380,384 to 1,381,886
_06235
Group
Condition
AO356
_06215
AO356
_06220
AO356
_06225
AO356
_06230
AO356
_06235
carbon source
L-Lysine (C)
-0.2
-0.1
-0.3
-0.6
N.D.
carbon source
L-Lysine (C)
-0.2
-0.0
-0.2
-0.5
N.D.
survival
4C survival; 1152 hrs
+0.1
+0.1
-0.4
-0.7
N.D.
pH
Minimal media pH6
-0.3
-0.1
-0.4
-0.0
N.D.
carbon source
Ethanol (C)
-0.1
-0.2
-0.5
+0.1
N.D.
stress
Ceftazidime 0.2 mg/ml
+0.1
+0.1
-0.5
-0.3
N.D.
stress
Lomefloxacin 0.0005 mM
+0.2
-0.0
-0.6
-0.2
N.D.
carbon source
L-Histidine (C)
-0.4
-0.0
+0.2
-0.3
N.D.
stress
perchlorate 75 mM
-0.2
+0.0
+0.1
-0.4
N.D.
stress
1-ethyl-3-methylimidazolium chloride 200 mM
+0.1
+0.2
-0.7
-0.1
N.D.
stress
Paraquat dichloride 0.0625 mg/ml
+0.4
-0.2
-0.2
-0.3
N.D.
stress
copper (II) chloride 2.5 mM
+0.2
+0.2
+0.2
-0.9
N.D.
anaerobic
Nitrate reduction with yeast extract
-0.4
+0.3
+0.2
-0.3
N.D.
carbon source
L-Arabinose (C)
-0.4
-0.0
+0.2
+0.2
N.D.
stress
nitrite 10 mM
-0.2
-0.2
+0.1
+0.3
N.D.
motility
outer cut, LB soft agar motility assay
+0.4
-0.0
-0.3
-0.1
N.D.
carbon source
Carbon source D-Mannitol 10 mM
+0.3
+0.1
-0.4
+0.1
N.D.
stress
methylglyoxal 0.016 vol%
-0.3
+0.3
+0.2
-0.1
N.D.
nitrogen source
L-tyrosine (N)
-0.4
+0.1
+0.3
+0.1
N.D.
carbon source
acetate (C)
-0.2
-0.1
+0.2
+0.3
N.D.
carbon source
Carbon source D,L-Lactate 10 mM
+0.3
-0.1
-0.3
+0.3
N.D.
stress
Vanillin 2.5 mM
-0.4
+0.1
+0.4
+0.1
N.D.
lb
LB
+0.2
+0.2
-0.3
+0.2
N.D.
stress
Sisomicin 0.001 mg/ml
+0.3
+0.1
-0.4
+0.3
N.D.
carbon source
D-Lactate (C)
+0.1
-0.1
+0.5
+0.2
N.D.
carbon source
a-Ketoglutaric (C)
+0.3
-0.1
+0.3
+0.3
N.D.
carbon source
D-Ribose (C)
+0.2
+0.1
+0.4
+0.2
N.D.
motility
outer cut, LB soft agar motility assay
+0.4
-0.2
+0.3
+0.4
N.D.
stress
Nickel (II) chloride 0.625 mM
+0.0
+0.2
+0.4
+0.4
N.D.
nitrogen source
Gly-Glu (N)
-0.3
+0.2
+0.4
+1.4
N.D.
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