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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_06105 and AO356_06110 are separated by 234 nucleotides
AO356_06110 and AO356_06115 are separated by 187 nucleotides
AO356_06115 and AO356_06120 are separated by 94 nucleotides
AO356_06120 and AO356_06125 are separated by 33 nucleotides
AO356_06105: AO356_06105 - cold-shock protein, at 1,353,859 to 1,354,068
_06105
AO356_06110: AO356_06110 - glycerol-3-phosphate O-acyltransferase, at 1,354,303 to 1,356,801
_06110
AO356_06115: AO356_06115 - hypothetical protein, at 1,356,989 to 1,357,360
_06115
AO356_06120: AO356_06120 - hypothetical protein, at 1,357,455 to 1,357,850
_06120
AO356_06125: AO356_06125 - hypothetical protein, at 1,357,884 to 1,358,573
_06125
Group
Condition
AO356
_06105
AO356
_06110
AO356
_06115
AO356
_06120
AO356
_06125
stress
Sisomicin 0.001 mg/ml
-2.1
-0.2
-0.0
+0.2
-0.0
carbon source
Carbon source D-Mannitol 10 mM
-1.6
+0.1
-0.2
-0.2
-0.1
stress
Lomefloxacin 0.0005 mM
-1.6
+0.1
-0.2
+0.1
-0.3
phage
P5ss-5 phage 10-1 dilution SM calcl2
-1.2
-0.2
-0.5
-0.3
+0.4
survival
4C survival; 192 hrs
-1.6
-0.1
-0.3
+0.0
+0.2
carbon source
Fumarate (C)
-0.6
+0.2
-1.1
-0.1
-0.0
stress
nitrate 200 mM
-1.4
+0.1
-0.3
-0.2
+0.2
stress
Thallium(I) acetate 0.008 mg/ml
-1.1
-0.0
-0.4
+0.2
-0.1
carbon source
D-Galactose (C)
-0.9
+0.1
-0.5
-0.0
-0.0
carbon source
L-Arabinose (C)
-0.6
+0.1
-0.6
-0.1
+0.1
stress
Nickel (II) chloride 0.625 mM
+0.3
-0.1
-0.7
-0.2
-0.2
carbon source
Carbon source D,L-Lactate 10 mM
-0.8
+0.1
-0.5
+0.1
+0.2
stress
Aluminum chloride 5 mM
-1.5
-0.1
+0.5
+0.1
+0.2
stress
Cholin acetate 40 mM
+0.4
+0.1
-0.9
+0.1
-0.3
stress
Rifampicin 0.0016 mg/ml
-0.9
+0.1
+0.3
+0.1
-0.1
stress
copper (II) chloride 2.5 mM
+0.4
-0.0
-0.8
+0.3
-0.0
pH
Minimal media pH6
-0.5
+0.0
+0.6
-0.3
-0.1
motility
outer cut, LB soft agar motility assay
-0.2
+0.2
+0.3
+0.2
-0.6
resistance
20 mM putrescine
+0.9
+0.1
-0.5
-0.1
-0.4
carbon source
Carbon source Sucrose 5 mM
+0.7
+0.1
-0.7
-0.1
+0.0
stress
KB-half, Pseudo5 supernatant
+0.9
-0.2
+0.1
-0.5
-0.1
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
+0.3
-0.2
+1.1
-0.6
-0.5
nitrogen source
Carnitine Hydrochloride (N)
+0.7
+0.2
-0.6
-0.1
-0.0
carbon source
Putrescine (C)
+0.9
+0.1
-0.6
-0.1
+0.1
stress
Benzalkonium Chloride 0.008 mg/ml
-0.8
+0.1
+1.3
+0.1
-0.1
carbon source
L-Lysine (C)
+1.1
-0.1
+0.2
-0.3
-0.2
stress
Spectinomycin 0.025 mg/ml
+0.9
-0.0
+0.3
-0.2
-0.2
carbon source
Carbon source D-Glucose 10 mM
+0.8
+0.2
+0.2
-0.0
-0.3
carbon source
L-Leucine (C)
+0.8
+0.1
+0.5
-0.1
+0.1
motility
outer cut, LB soft agar motility assay
-0.2
+0.2
+0.5
+0.3
+0.9
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