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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_05500 and AO356_05505 overlap by 4 nucleotides
AO356_05505 and AO356_05510 are separated by 27 nucleotides
AO356_05510 and AO356_05515 are separated by 13 nucleotides
AO356_05515 and AO356_05520 are separated by 220 nucleotides
AO356_05500: AO356_05500 - ABC transporter for L-Lysine, permease component 1
(from data)
, at 1,232,990 to 1,233,718
_05500
AO356_05505: AO356_05505 - ABC transporter for L-Lysine, permease component 2
(from data)
, at 1,233,715 to 1,234,425
_05505
AO356_05510: AO356_05510 - succinylglutamate desuccinylase, at 1,234,453 to 1,235,571
_05510
AO356_05515: AO356_05515 - ABC transporter for L-Lysine, ATPase component
(from data)
, at 1,235,585 to 1,236,349
_05515
AO356_05520: AO356_05520 - DNA ligase-associated DEXH box helicase, at 1,236,570 to 1,239,056
_05520
Group
Condition
AO356
_05500
AO356
_05505
AO356
_05510
AO356
_05515
AO356
_05520
carbon source
L-Lysine (C)
-2.5
-2.2
-1.3
-2.1
+0.1
carbon source
L-Lysine (C)
-2.1
-1.9
-1.3
-2.4
+0.2
carbon source
L-Histidine (C)
-0.6
-0.7
+0.1
-1.1
+0.1
carbon source
L-Histidine (C)
-0.4
-0.5
-0.3
-0.8
+0.1
stress
Chlorite 0.04 mM
-0.2
-0.2
-0.1
-0.7
-0.1
carbon source
L-Arginine (C)
-0.3
-0.3
-0.2
-0.5
-0.0
carbon source
L-Arginine (C)
-0.4
-0.3
-0.2
-0.3
+0.0
nitrogen source
Gly-Glu (N)
-0.3
-0.2
-0.4
-0.3
+0.1
stress
Sisomicin 0.001 mg/ml
-0.2
-0.4
+0.3
-0.7
-0.1
stress
Bacitracin 2 mg/ml
-0.0
-0.1
-0.2
-0.4
-0.3
motility
outer cut, LB soft agar motility assay
+0.2
+0.1
-0.6
-0.7
+0.2
anaerobic
Nitrate reduction with yeast extract
-0.3
+0.0
-1.0
+0.5
-0.0
stress
copper (II) chloride 2.5 mM
+0.3
-0.1
-0.9
+0.2
-0.1
stress
nitrite 10 mM
+0.2
-0.1
+0.4
-0.9
-0.3
nitrogen source
Uridine (N)
-0.3
-0.0
-0.5
+0.3
-0.0
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
-0.1
-0.0
-0.6
-0.2
+0.7
stress
Cholin acetate 20 mM
+0.2
-0.1
-0.5
+0.3
-0.1
carbon source
L-Malic (C)
+0.1
-0.0
-0.5
+0.3
+0.0
survival
4C survival; 360 hrs
-0.3
-0.1
+0.5
+0.0
-0.1
stress
KB-half, Pseudo5 supernatant
-0.1
+0.1
-0.4
+0.3
+0.3
carbon source
L-Alanine (C)
+0.1
+0.2
+0.1
+0.4
-0.2
nitrogen source
L-Isoleucine (N)
+0.0
+0.1
+0.4
+0.3
-0.2
nitrogen source
L-Leucine (N)
-0.1
-0.1
+0.3
+0.3
+0.3
carbon source
acetate (C)
-0.0
+0.1
+0.2
+0.1
+0.5
carbon source
D-Alanine (C)
+0.2
+0.1
+0.3
+0.4
-0.1
carbon source
Ethanol (C)
+0.3
+0.2
+0.6
-0.1
+0.1
motility
outer cut, LB soft agar motility assay
+0.3
-0.0
+0.6
+0.2
-0.1
carbon source
Ethanol (C)
+0.2
+0.2
+0.7
+0.0
-0.0
motility
outer cut, LB soft agar motility assay
-0.0
+0.2
+0.5
+0.2
+0.2
carbon source
succinate (C)
+0.2
+0.0
+0.3
+0.5
+0.1
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