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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_05475 and AO356_05480 are separated by 207 nucleotides
AO356_05480 and AO356_05485 are separated by 234 nucleotides
AO356_05485 and AO356_05490 overlap by 4 nucleotides
AO356_05490 and AO356_05495 are separated by 325 nucleotides
AO356_05475: AO356_05475 - isochorismatase, at 1,225,658 to 1,226,215
_05475
AO356_05480: AO356_05480 - acyl-homoserine lactone acylase subunit beta, at 1,226,423 to 1,228,873
_05480
AO356_05485: AO356_05485 - DNA ligase-associated DEXH box helicase, at 1,229,108 to 1,230,139
_05485
AO356_05490: AO356_05490 - ATP-dependent DNA ligase, at 1,230,136 to 1,231,824
_05490
AO356_05495: AO356_05495 - ABC transporter for L-Lysine, periplasmic substrate-binding component
(from data)
, at 1,232,150 to 1,232,932
_05495
Group
Condition
AO356
_05475
AO356
_05480
AO356
_05485
AO356
_05490
AO356
_05495
carbon source
L-Lysine (C)
-0.3
+0.3
+0.4
+0.4
-3.0
carbon source
L-Lysine (C)
-0.1
+0.2
+0.3
+0.7
-2.3
nitrogen source
L-Phenylalanine (N)
-0.8
+0.0
-0.0
-0.0
-0.1
stress
Aluminum chloride 5 mM
-0.8
-0.2
-0.0
+0.0
+0.1
carbon source
Carbon source D-Mannitol 2.5 mM
-0.7
-0.0
+0.1
-0.0
-0.1
nitrogen source
L-Isoleucine (N)
-0.5
+0.0
-0.1
-0.1
+0.1
motility
outer cut, LB soft agar motility assay
-0.8
-0.1
+0.1
+0.1
+0.1
stress
nitrite 10 mM
-0.6
+0.1
+0.0
-0.2
+0.1
survival
4C survival; 672 hrs
-0.6
+0.0
-0.0
+0.2
-0.2
carbon source
D-Alanine (C)
-0.6
+0.2
+0.0
-0.1
-0.1
stress
Benzalkonium Chloride 0.008 mg/ml
-0.4
+0.2
-0.1
-0.1
-0.1
stress
Cholin acetate 40 mM
-0.5
-0.2
+0.2
-0.0
+0.0
carbon source
Ethanol (C)
-0.6
-0.0
-0.0
-0.0
+0.2
stress
Benzalkonium Chloride 0.016 mg/ml
+0.2
-0.3
-0.1
-0.2
-0.1
survival
4C survival; 1152 hrs
-0.4
+0.0
-0.2
+0.3
-0.1
carbon source
L-Histidine (C)
-0.0
+0.2
+0.1
-0.2
-0.5
carbon source
L-Histidine (C)
+0.0
+0.3
+0.1
-0.3
-0.5
anaerobic
Nitrate reduction with yeast extract
+0.6
-0.1
-0.1
+0.0
-0.7
phage
P5ss-5 phage 10-1 dilution SM calcl2
+0.8
+0.0
-0.3
+0.1
-0.7
carbon source
a-Ketoglutaric (C)
-0.4
+0.1
+0.0
+0.1
+0.2
survival
4C survival; 360 hrs
-0.4
+0.1
+0.2
+0.3
-0.2
carbon source
D-Glucose (C)
+0.3
+0.2
-0.2
-0.2
-0.0
motility
outer cut, LB soft agar motility assay
+0.4
-0.2
+0.1
-0.3
+0.4
motility
outer cut, LB soft agar motility assay
+0.5
-0.2
-0.4
+0.4
+0.3
carbon source
D-Ribose (C)
+0.4
+0.4
-0.0
-0.3
+0.0
carbon source
D-Xylose (C)
+0.3
+0.4
-0.1
-0.1
+0.1
stress
Vanillin 2.5 mM
+0.6
+0.1
-0.1
-0.1
+0.3
stress
1-ethyl-3-methylimidazolium chloride 200 mM
+0.2
+0.2
+0.2
+0.0
+0.2
carbon source
L-Malic (C)
+0.4
+0.1
+0.1
+0.2
+0.0
nitrogen source
nitrite (N)
+0.3
+0.2
+0.1
+0.4
+0.1
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