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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_05450 and AO356_05455 are separated by 192 nucleotides
AO356_05455 and AO356_05460 are separated by 2 nucleotides
AO356_05460 and AO356_05465 are separated by 70 nucleotides
AO356_05465 and AO356_05470 are separated by 5 nucleotides
AO356_05450: AO356_05450 - hypothetical protein, at 1,222,883 to 1,223,578
_05450
AO356_05455: AO356_05455 - hypothetical protein, at 1,223,771 to 1,224,268
_05455
AO356_05460: AO356_05460 - hypothetical protein, at 1,224,271 to 1,224,759
_05460
AO356_05465: AO356_05465 - hypothetical protein, at 1,224,830 to 1,225,315
_05465
AO356_05470: AO356_05470 - zinc chelation protein SecC, at 1,225,321 to 1,225,524
_05470
Group
Condition
AO356
_05450
AO356
_05455
AO356
_05460
AO356
_05465
AO356
_05470
motility
outer cut, LB soft agar motility assay
-0.2
-0.0
-0.1
-1.0
N.D.
anaerobic
Nitrate reduction with yeast extract
+0.2
-0.6
-0.1
-0.9
N.D.
stress
Nickel (II) chloride 0.625 mM
-0.0
-0.9
+0.0
-0.5
N.D.
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
-0.4
-0.2
-0.3
-0.3
N.D.
carbon source
D-Glucosamine Hydrochloride (C)
-0.1
-0.2
-0.0
-0.7
N.D.
stress
1-ethyl-3-methylimidazolium chloride 200 mM
-0.4
-0.3
+0.0
-0.5
N.D.
survival
4C survival; 1152 hrs
-0.2
+0.1
-0.4
-0.6
N.D.
stress
Lomefloxacin 0.0005 mM
-0.2
-0.2
+0.0
-0.6
N.D.
carbon source
L-Lactate (C)
-0.1
-0.6
+0.0
-0.4
N.D.
stress
Cisplatin 0.0032 mg/ml
-0.1
-0.6
-0.1
-0.2
N.D.
lb
LB
+0.0
-0.6
-0.1
-0.2
N.D.
pH
Minimal media pH7
-0.2
-0.4
-0.1
-0.2
N.D.
carbon source
D-Mannose (C)
-0.0
-0.5
+0.1
-0.4
N.D.
carbon source
Carbon source D-Mannitol 10 mM
-0.2
-0.4
+0.2
-0.5
N.D.
carbon source
D,L-Lactate (C)
-0.1
-0.6
+0.1
-0.2
N.D.
motility
outer cut, LB soft agar motility assay
+0.1
-0.3
-0.0
-0.6
N.D.
carbon source
D-Gluconic Acid (C)
-0.1
-0.7
+0.1
-0.1
N.D.
carbon source
pyruvate (C)
-0.1
-0.4
+0.1
-0.3
N.D.
carbon source
D-Xylose (C)
-0.1
-1.3
+0.5
+0.2
N.D.
carbon source
L-Histidine (C)
-0.1
-0.7
+0.1
+0.2
N.D.
phage
P5ss-5 phage 10-1 dilution SM calcl2
-0.2
+0.4
+0.0
-0.7
N.D.
carbon source
D-Xylose (C)
+0.0
-0.6
+0.5
-0.1
N.D.
stress
Rifampicin 0.0016 mg/ml
+0.2
+0.2
+0.0
-0.6
N.D.
carbon source
acetate (C)
-0.0
-0.7
+0.2
+0.4
N.D.
stress
Sisomicin 0.001 mg/ml
-0.2
-0.1
-0.2
+0.5
N.D.
nitrogen source
nitrite (N)
-0.1
-0.4
+0.1
+0.5
N.D.
stress
Benzalkonium Chloride 0.016 mg/ml
-0.2
+0.6
+0.0
-0.2
N.D.
motility
outer cut, LB soft agar motility assay
-0.4
+0.7
+0.0
-0.0
N.D.
stress
Paraquat dichloride 0.0625 mg/ml
-0.1
+0.3
+0.2
+0.4
N.D.
motility
outer cut, LB soft agar motility assay
-0.1
+0.4
+0.1
+0.5
N.D.
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