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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_05045 and AO356_05050 are separated by 208 nucleotides
AO356_05050 and AO356_05055 are separated by 150 nucleotides
AO356_05055 and AO356_05060 are separated by 92 nucleotides
AO356_05060 and AO356_05065 are separated by 216 nucleotides
AO356_05045: AO356_05045 - lysine transporter LysE, at 1,134,845 to 1,135,507
_05045
AO356_05050: AO356_05050 - glutamate-pyruvate aminotransferase, at 1,135,716 to 1,136,933
_05050
AO356_05055: AO356_05055 - permease DsdX, at 1,137,084 to 1,138,436
_05055
AO356_05060: AO356_05060 - gluconokinase, at 1,138,529 to 1,139,062
_05060
AO356_05065: AO356_05065 - LacI family transcriptional regulator, at 1,139,279 to 1,140,310
_05065
Group
Condition
AO356
_05045
AO356
_05050
AO356
_05055
AO356
_05060
AO356
_05065
nitrogen source
D-Serine (N)
+0.4
-0.3
-4.3
-4.9
+0.9
pH
Minimal media pH8
-0.2
+0.1
-3.6
-3.5
+0.2
pH
Minimal media pH7
-0.2
+0.1
-2.9
-2.7
-0.1
carbon source
Carbon source D-Glucose 10 mM
-0.0
+0.1
-1.6
-3.2
-0.1
pH
Minimal media pH6
-0.2
+0.2
-2.3
-2.8
+0.3
nitrogen source
nitrite (N)
-0.0
+0.1
-2.5
-2.3
+0.0
pH
Minimal media pH6
-0.1
-0.1
-2.4
-1.7
+0.2
pH
Minimal media pH7
+0.0
+0.1
-2.1
-1.9
-0.1
carbon source
Carbon source D-Glucose 10 mM
-0.1
-0.0
-2.0
-1.6
-0.1
pH
Minimal media pH8
-0.0
+0.1
-2.0
-2.0
+0.3
carbon source
D-Glucose (C)
+0.3
+0.1
-2.1
-1.4
-0.0
carbon source
L-Histidine (C)
-0.1
-0.0
+0.1
-0.3
-2.7
nitrogen source
Urea (N)
+0.2
+0.0
-1.3
-1.9
+0.1
nitrogen source
L-Isoleucine (N)
+0.2
+0.1
-2.0
-1.9
+0.7
carbon source
D-Glucose (C)
-0.0
-0.2
-2.0
-0.5
-0.0
carbon source
L-Histidine (C)
+0.2
-0.1
+0.2
-0.4
-2.2
nitrogen source
Ammonium chloride (N)
+0.2
+0.2
-1.4
-1.1
-0.2
motility
outer cut, LB soft agar motility assay
-0.1
+0.2
-1.2
-1.0
-0.1
carbon source
L-Lysine (C)
+0.0
+0.2
+0.0
-1.2
-1.0
nitrogen source
D-Alanine (N)
+0.1
-0.0
-0.8
-1.1
+0.1
nitrogen source
Adenine (N)
-0.0
+0.1
-1.1
-0.7
-0.1
nitrogen source
D-Glucosamine Hydrochloride (N)
+0.1
-0.0
-0.7
-0.9
+0.0
nitrogen source
L-Leucine (N)
+0.2
+0.1
-0.7
-1.0
+0.2
carbon source
L-Lysine (C)
-0.1
+0.2
+0.4
-0.6
-1.0
motility
outer cut, LB soft agar motility assay
+0.2
-0.6
+0.4
-0.7
-0.0
stress
KB-half, Pseudo5 supernatant
+0.2
-0.0
-0.4
-0.9
+0.4
resistance
50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
+0.2
-0.5
-0.2
+0.4
-0.6
carbon source
D-Gluconic Acid (C)
+0.1
+0.3
+0.4
+0.8
-0.5
carbon source
Carbon source Sucrose 10 mM
+0.3
+0.1
+0.7
+0.7
-0.1
carbon source
D-Glucosamine Hydrochloride (C)
+0.1
+0.1
-1.0
+1.4
+4.4
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