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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_04520 and AO356_04525 are separated by 4 nucleotides
AO356_04525 and AO356_04530 are separated by 17 nucleotides
AO356_04530 and AO356_04535 are separated by 315 nucleotides
AO356_04535 and AO356_04540 are separated by 10 nucleotides
AO356_04520: AO356_04520 - MBL fold metallo-hydrolase, at 1,020,635 to 1,021,393
_04520
AO356_04525: AO356_04525 - hypothetical protein, at 1,021,398 to 1,022,513
_04525
AO356_04530: AO356_04530 - 4-hydroxy-tetrahydrodipicolinate synthase, at 1,022,531 to 1,023,409
_04530
AO356_04535: AO356_04535 - glycine cleavage system protein R, at 1,023,725 to 1,024,285
_04535
AO356_04540: AO356_04540 - peroxiredoxin, at 1,024,296 to 1,024,769
_04540
Group
Condition
AO356
_04520
AO356
_04525
AO356
_04530
AO356
_04535
AO356
_04540
pH
Minimal media pH9
-0.2
-3.1
N.D.
-0.5
-0.2
stress
perchlorate 75 mM
-0.1
-1.9
N.D.
-0.7
-0.3
stress
copper (II) chloride 2.5 mM
-0.2
-0.8
N.D.
-0.6
-0.8
carbon source
Putrescine (C)
-0.0
-1.2
N.D.
-0.7
-0.4
carbon source
D-Alanine (C)
-0.3
-0.9
N.D.
-0.6
-0.2
stress
Lomefloxacin 0.0005 mM
-0.2
-0.8
N.D.
-0.4
-0.5
nitrogen source
Uridine (N)
-0.1
-0.5
N.D.
-1.3
+0.0
carbon source
Ethanol (C)
-0.1
-0.9
N.D.
-0.4
-0.4
stress
Rifampicin 0.0016 mg/ml
-0.1
-0.8
N.D.
-0.5
-0.4
survival
4C survival; 360 hrs
-0.1
-1.4
N.D.
-0.1
-0.1
carbon source
Carbon source D-Mannitol 2.5 mM
-0.2
-0.7
N.D.
-0.7
-0.1
carbon source
Ethanol (C)
+0.1
-0.6
N.D.
-0.3
-0.8
phage
P5ss-5 phage 10-1 dilution SM calcl2
+0.0
-1.2
N.D.
-0.2
-0.2
carbon source
L-Leucine (C)
-0.1
-0.7
N.D.
-0.5
-0.2
carbon source
m-Inositol (C)
-0.2
-0.9
N.D.
-0.4
+0.0
survival
4C survival; 1152 hrs
-0.2
-1.1
N.D.
-0.3
+0.1
survival
4C survival; 192 hrs
+0.0
-1.4
N.D.
+0.1
-0.1
stress
Rifampicin 0.0024 mg/ml
+0.1
-0.6
N.D.
-0.8
-0.1
stress
nitrite 10 mM
+0.0
-0.4
N.D.
-0.7
-0.3
stress
1-ethyl-3-methylimidazolium lysine 10 mM
-0.3
-1.4
N.D.
+0.4
-0.0
carbon source
Carbon source Sucrose 5 mM
-0.2
-1.1
N.D.
-0.0
+0.2
stress
Benzalkonium Chloride 0.016 mg/ml
+0.0
+0.5
N.D.
-1.8
+0.0
anaerobic
Nitrate reduction with yeast extract
-0.1
-1.3
N.D.
+0.4
+0.2
stress
nitrate 200 mM
+0.2
-0.7
N.D.
-0.5
+0.2
motility
outer cut, LB soft agar motility assay
-0.5
-0.5
N.D.
-0.1
+0.4
nitrogen source
Urea (N)
-0.6
+0.5
N.D.
-0.7
+0.2
motility
outer cut, LB soft agar motility assay
+0.2
+0.3
N.D.
-1.1
-0.0
carbon source
acetate (C)
-0.1
-0.8
N.D.
+0.6
-0.0
carbon source
acetate (C)
-0.2
-0.8
N.D.
+0.6
+0.2
stress
Choline chloride 500 mM
+0.2
+0.2
N.D.
+0.9
+0.2
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