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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_04335 and AO356_04340 are separated by 64 nucleotides
AO356_04340 and AO356_04345 are separated by 318 nucleotides
AO356_04345 and AO356_04350 are separated by 12 nucleotides
AO356_04350 and AO356_04355 are separated by 32 nucleotides
AO356_04335: AO356_04335 - hypothetical protein, at 986,833 to 987,585
_04335
AO356_04340: AO356_04340 - MFS transporter, at 987,650 to 988,936
_04340
AO356_04345: AO356_04345 - hypothetical protein, at 989,255 to 989,797
_04345
AO356_04350: AO356_04350 - glutamine amidotransferase, at 989,810 to 990,586
_04350
AO356_04355: AO356_04355 - MFS transporter, at 990,619 to 991,116
_04355
Group
Condition
AO356
_04335
AO356
_04340
AO356
_04345
AO356
_04350
AO356
_04355
carbon source
L-Histidine (C)
-4.8
-0.3
-0.4
-0.4
-0.3
anaerobic
Nitrate reduction with yeast extract
+0.2
-0.1
-3.2
-0.2
-2.3
carbon source
L-Histidine (C)
-4.6
-0.2
+0.0
-0.2
+0.2
motility
outer cut, LB soft agar motility assay
-0.1
+0.0
-3.7
-0.4
+0.2
phage
P5ss-5 phage 10-1 dilution SM calcl2
+0.1
-0.4
-0.8
-0.3
-2.3
motility
outer cut, LB soft agar motility assay
-0.2
+0.1
-2.9
-0.4
-0.4
motility
outer cut, LB soft agar motility assay
-0.8
-0.2
-1.2
-0.2
-0.8
survival
4C survival; 1152 hrs
-1.2
-0.0
-0.7
-0.2
-1.1
survival
4C survival; 672 hrs
-0.8
-0.1
-0.4
-0.6
-1.1
stress
Choline chloride 500 mM
-0.1
-0.1
+0.1
-0.3
-2.4
pH
Minimal media pH9
-1.8
+0.1
+0.0
-0.2
-0.5
stress
perchlorate 75 mM
+0.1
-0.3
-0.0
-0.1
-1.6
carbon source
L-Lysine (C)
-1.5
-0.2
+0.1
+0.0
-0.2
stress
Sisomicin 0.001 mg/ml
-1.9
+0.1
+0.4
+0.0
-0.3
carbon source
D-Lactate (C)
-2.1
-0.0
+0.2
+0.1
+0.1
carbon source
L-Lysine (C)
-1.6
-0.1
-0.1
-0.0
+0.3
stress
Nickel (II) chloride 0.625 mM
-0.0
+0.2
-0.3
-0.6
-0.8
carbon source
D-Lactate (C)
-1.4
-0.2
+0.3
-0.2
+0.1
carbon source
L-Proline (C)
-1.1
-0.2
-0.3
+0.1
+0.3
stress
Benzethonium chloride 0.025 mM
+0.1
+0.1
-0.8
+0.1
-0.7
carbon source
L-Isoleucine (C)
-0.7
-0.0
+0.2
+0.1
-0.7
carbon source
L-Glutamine (C)
-0.9
-0.3
-0.2
-0.2
+0.4
motility
outer cut, LB soft agar motility assay
-0.4
+0.3
-1.0
-0.3
+0.5
stress
Aluminum chloride 5 mM
+0.2
-0.1
+0.5
-0.2
-1.0
carbon source
Carbon source D-Mannitol 2.5 mM
-0.3
-0.2
+0.7
+0.1
-0.7
stress
1-ethyl-3-methylimidazolium chloride 200 mM
+0.2
-0.1
-0.1
+0.5
-1.0
stress
Rifampicin 0.0024 mg/ml
-0.2
-0.0
-0.9
-0.1
+1.2
carbon source
D-Fructose (C)
-0.7
-0.1
+0.3
+0.2
+0.5
nitrogen source
D-Glucosamine Hydrochloride (N)
-0.6
-0.1
+0.4
+0.1
+0.6
carbon source
L-Leucine (C)
+0.6
-0.1
+0.8
-0.2
+0.1
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