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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_04155 and AO356_04160 are separated by 263 nucleotides
AO356_04160 and AO356_04165 are separated by 44 nucleotides
AO356_04165 and AO356_04170 are separated by 206 nucleotides
AO356_04170 and AO356_04175 are separated by 501 nucleotides
AO356_04155: AO356_04155 - thioredoxin, at 934,102 to 934,833
_04155
AO356_04160: AO356_04160 - hypothetical protein, at 935,097 to 935,369
_04160
AO356_04165: AO356_04165 - hypothetical protein, at 935,414 to 935,974
_04165
AO356_04170: AO356_04170 - hypothetical protein, at 936,181 to 936,360
_04170
AO356_04175: AO356_04175 - hypothetical protein, at 936,862 to 937,338
_04175
Group
Condition
AO356
_04155
AO356
_04160
AO356
_04165
AO356
_04170
AO356
_04175
stress
KB-half, Pseudo5 supernatant
+0.0
-1.0
-0.3
N.D.
-0.4
motility
outer cut, LB soft agar motility assay
+0.1
-1.6
+0.2
N.D.
-0.3
carbon source
L-Leucine (C)
+0.1
-0.8
-0.1
N.D.
-0.3
pH
Minimal media pH9
+0.0
-0.6
-0.1
N.D.
-0.3
carbon source
Ethanol (C)
-0.1
-0.9
+0.0
N.D.
-0.0
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
+0.0
-0.4
-0.4
N.D.
-0.3
stress
Paraquat dichloride 0.0625 mg/ml
-0.1
-0.9
-0.1
N.D.
+0.2
stress
1-ethyl-3-methylimidazolium acetate 20 mM
+0.1
-0.5
-0.4
N.D.
+0.0
carbon source
D-Alanine (C)
+0.1
-0.5
+0.1
N.D.
-0.2
nitrogen source
D-Serine (N)
-0.0
-0.3
+0.4
N.D.
-0.5
stress
Nickel (II) chloride 0.625 mM
+0.1
-0.5
-0.2
N.D.
+0.2
stress
Sisomicin 0.001 mg/ml
-0.3
+0.4
-0.1
N.D.
-0.3
carbon source
Ethanol (C)
+0.0
-0.7
+0.0
N.D.
+0.2
survival
4C survival; 360 hrs
-0.1
-0.5
-0.1
N.D.
+0.4
motility
outer cut, LB soft agar motility assay
-0.1
+0.4
-0.6
N.D.
-0.0
stress
nitrate 200 mM
+0.3
-0.1
-0.5
N.D.
-0.0
carbon source
Carbon source Sucrose 5 mM
-0.0
+0.4
-0.5
N.D.
-0.0
resistance
10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
-0.2
-0.5
+0.3
N.D.
+0.3
stress
1-ethyl-3-methylimidazolium chloride 200 mM
+0.3
-0.3
-0.4
N.D.
+0.5
stress
Cholin acetate 40 mM
-0.1
+0.2
-0.4
N.D.
+0.5
motility
outer cut, LB soft agar motility assay
+0.3
-0.7
+0.5
N.D.
+0.0
stress
1-ethyl-3-methylimidazolium acetate 10 mM
+0.1
+0.3
-0.3
N.D.
+0.2
anaerobic
Nitrate reduction with yeast extract
-0.0
+1.1
-0.3
N.D.
-0.5
carbon source
Carbon source D-Mannitol 5 mM
+0.1
+0.7
-0.0
N.D.
-0.4
carbon source
D-Lactate (C)
-0.0
+0.6
-0.3
N.D.
+0.2
carbon source
Fumarate (C)
-0.1
+0.5
-0.1
N.D.
+0.4
nitrogen source
Thymine (N)
+0.1
+0.6
+0.2
N.D.
-0.2
stress
Ceftazidime 0.2 mg/ml
-0.1
+0.5
-0.0
N.D.
+0.3
motility
outer cut, LB soft agar motility assay
+0.0
+1.1
+0.4
N.D.
-0.3
stress
methylglyoxal 0.016 vol%
+0.2
+0.9
+0.1
N.D.
+0.3
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