Fitness for 5 genes in Pseudomonas fluorescens FW300-N2C3

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500 ntAO356_03835 and AO356_03840 are separated by 15 nucleotidesAO356_03840 and AO356_03845 are separated by 223 nucleotidesAO356_03845 and AO356_03850 are separated by 5 nucleotidesAO356_03850 and AO356_03855 are separated by 110 nucleotides AO356_03835: AO356_03835 - flagellar M-ring protein FliF, at 848,338 to 850,122 _03835 AO356_03840: AO356_03840 - flagellar hook-basal body protein FliE, at 850,138 to 850,464 _03840 AO356_03845: AO356_03845 - Fis family transcriptional regulator, at 850,688 to 852,082 _03845 AO356_03850: AO356_03850 - histidine kinase, at 852,088 to 853,296 _03850 AO356_03855: AO356_03855 - AAA family ATPase, at 853,407 to 854,882 _03855
Group Condition AO356_03835 AO356_03840 AO356_03845 AO356_03850 AO356_03855
motility outer cut, LB soft agar motility assay -3.2 -3.3 -0.9 -1.1 -4.7
motility outer cut, LB soft agar motility assay -3.2 -3.5 -0.6 -0.6 -3.1
motility outer cut, LB soft agar motility assay -2.7 -2.2 -0.8 -0.8 -3.7
survival 4C survival; 1152 hrs -2.2 -1.9 -1.5 -0.5 -2.4
motility outer cut, LB soft agar motility assay -2.4 -1.9 -0.4 -0.5 -2.8
survival 4C survival; 672 hrs -1.6 -1.6 -0.8 -0.5 -2.1
survival 4C survival; 360 hrs -1.3 -1.3 -0.5 +0.0 -1.7
stress Cobalt chloride 0.08 mM +0.2 +0.2 +0.1 +0.3 +2.9
resistance 10% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine +0.8 +0.8 +0.5 +0.4 +1.1
resistance 50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine +0.9 +0.9 +0.2 +0.5 +1.2
carbon source D-Fructose (C) +0.7 +0.7 +0.4 +0.4 +1.7
pH Minimal media pH6 +1.3 +1.4 +0.3 +0.2 +0.6
carbon source D-Galactose (C) +0.7 +0.7 +0.4 +0.4 +1.6
stress Spectinomycin 0.025 mg/ml +0.5 +0.3 +0.2 +0.1 +3.0
carbon source Putrescine (C) +1.1 +0.9 +0.2 +0.5 +1.5
carbon source Cytidine (C) +1.1 +1.1 +0.4 +0.4 +1.2
carbon source L-Asparagine (C) +1.5 +1.3 +0.5 +0.2 +0.7
resistance 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine +1.0 +1.2 +0.6 +0.4 +1.4
resistance 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine +1.2 +1.1 +0.4 +0.6 +1.4
carbon source Uridine (C) +1.3 +1.1 +0.5 +0.7 +1.3
carbon source D-Ribose (C) +1.2 +1.3 +0.3 +0.4 +1.8
resistance 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine +1.3 +1.4 +0.6 +0.6 +2.0
carbon source D-Ribose (C) +1.3 +1.3 +0.7 +0.7 +2.3
nitrogen source D-Glucosamine Hydrochloride (N) +1.7 +1.9 +0.4 +0.4 +2.3
carbon source acetate (C) +1.7 +1.4 +0.9 +0.7 +2.4
carbon source acetate (C) +1.7 +1.7 +0.7 +0.7 +2.5
carbon source D-Xylose (C) +1.8 +1.8 +0.9 +0.9 +2.5
carbon source D-Trehalose (C) +2.5 +2.3 +1.2 +1.2 +1.4
carbon source D-Trehalose (C) +2.6 +2.3 +1.2 +1.3 +1.7
carbon source D-Xylose (C) +1.9 +2.1 +1.0 +0.9 +3.2
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