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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_03800 and AO356_03805 are separated by 31 nucleotides
AO356_03805 and AO356_03810 are separated by 5 nucleotides
AO356_03810 and AO356_03815 are separated by 102 nucleotides
AO356_03815 and AO356_03820 are separated by 6 nucleotides
AO356_03800: AO356_03800 - histidine kinase, at 842,173 to 842,517
_03800
AO356_03805: AO356_03805 - chemotaxis protein CheY, at 842,549 to 844,255
_03805
AO356_03810: AO356_03810 - anti-anti-sigma factor, at 844,261 to 844,566
_03810
AO356_03815: AO356_03815 - flagellar biogenesis protein, at 844,669 to 845,118
_03815
AO356_03820: AO356_03820 - flagellar protein export ATPase FliI, at 845,125 to 846,483
_03820
Group
Condition
AO356
_03800
AO356
_03805
AO356
_03810
AO356
_03815
AO356
_03820
motility
outer cut, LB soft agar motility assay
-2.6
+0.5
+1.4
-3.1
-3.9
nitrogen source
L-Isoleucine (N)
-2.1
+0.2
-5.1
+0.3
+0.2
carbon source
L-Histidine (C)
-3.3
-0.9
-2.7
+0.1
+0.4
carbon source
D-Glucosamine Hydrochloride (C)
-1.8
-0.1
-3.8
-0.4
-0.3
motility
outer cut, LB soft agar motility assay
-2.0
+0.5
+1.9
-3.0
-3.0
carbon source
Putrescine (C)
-4.0
-0.3
-2.7
+1.0
+1.0
nitrogen source
Adenosine (N)
-1.3
+0.3
-4.5
+0.3
+0.2
motility
outer cut, LB soft agar motility assay
+1.1
+0.5
+1.4
-3.9
-3.8
carbon source
L-Lysine (C)
-2.2
-0.5
-2.8
+0.3
+0.4
carbon source
Inosine (C)
-2.6
+0.1
-2.7
+0.3
+0.3
carbon source
L-Histidine (C)
-2.7
-0.6
-2.1
+0.4
+0.4
motility
outer cut, LB soft agar motility assay
-2.7
+0.6
+1.8
-1.8
-2.4
nitrogen source
Urea (N)
-1.8
+0.3
-3.4
+0.5
+0.4
carbon source
Cytidine (C)
+0.1
-0.5
-5.6
+1.0
+1.0
carbon source
L-Lysine (C)
-1.4
-0.6
-2.9
+0.5
+0.6
carbon source
D-Fructose (C)
-2.0
-0.1
-2.1
+0.6
+0.8
resistance
50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine
-2.6
-0.9
-0.8
+0.7
+0.9
carbon source
L-Asparagine (C)
-4.4
-0.0
-0.1
+1.0
+1.1
survival
4C survival; 672 hrs
-0.9
+0.1
+1.9
-1.8
-1.5
nitrogen source
D-Glucosamine Hydrochloride (N)
-0.8
+0.7
-5.6
+1.9
+1.8
pH
Minimal media pH6
-2.5
-0.4
-0.4
+1.1
+1.2
carbon source
Uridine (C)
-0.4
-0.2
-2.5
+1.1
+1.3
resistance
50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
-1.2
-1.3
-0.5
+1.3
+1.3
carbon source
L-Asparagine (C)
-2.0
-0.1
-0.7
+1.4
+1.4
carbon source
D-Xylose (C)
-2.4
-1.0
-0.7
+2.1
+2.2
carbon source
D-Ribose (C)
-0.3
-0.2
-2.3
+1.5
+1.4
carbon source
D-Xylose (C)
-1.0
-1.1
-0.9
+1.7
+2.0
survival
4C survival; 1152 hrs
-0.3
+0.1
+5.9
-1.4
-2.3
carbon source
D-Trehalose (C)
+2.8
-0.1
-0.4
+2.4
+2.4
carbon source
D-Trehalose (C)
+2.3
-0.2
+0.8
+2.4
+2.3
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