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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_03775 and AO356_03780 are separated by 0 nucleotides
AO356_03780 and AO356_03785 are separated by 71 nucleotides
AO356_03785 and AO356_03790 are separated by 9 nucleotides
AO356_03790 and AO356_03795 are separated by 231 nucleotides
AO356_03775: AO356_03775 - flagellar assembly protein FliO, at 838,030 to 838,467
_03775
AO356_03780: AO356_03780 - flagellar motor switch protein, at 838,468 to 838,926
_03780
AO356_03785: AO356_03785 - flagellar motor switch protein FliM, at 838,998 to 839,966
_03785
AO356_03790: AO356_03790 - flagellar basal body protein FliL, at 839,976 to 840,479
_03790
AO356_03795: AO356_03795 - flagellar hook-length control protein, at 840,711 to 842,084
_03795
Group
Condition
AO356
_03775
AO356
_03780
AO356
_03785
AO356
_03790
AO356
_03795
motility
outer cut, LB soft agar motility assay
-4.7
-1.6
-3.8
-0.2
-5.1
motility
outer cut, LB soft agar motility assay
-2.0
-4.4
-3.9
-0.5
-3.4
survival
4C survival; 1152 hrs
-4.0
-2.6
-2.4
-1.2
-1.8
motility
outer cut, LB soft agar motility assay
-1.5
-4.5
-2.7
-0.2
-2.5
motility
outer cut, LB soft agar motility assay
-2.5
-1.3
-2.8
-1.1
-3.3
survival
4C survival; 672 hrs
-2.7
-1.8
-1.5
-0.9
-1.4
survival
4C survival; 360 hrs
-1.2
-1.6
-1.0
-0.6
-1.4
survival
4C survival; 192 hrs
-1.5
-1.8
-0.7
-0.4
-0.8
resistance
50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine
+1.5
+0.7
+0.9
+0.3
+0.5
pH
Minimal media pH7
+1.3
+0.6
+0.8
+0.6
+0.7
resistance
20 mM putrescine
+1.6
+0.6
+0.9
+0.4
+0.5
carbon source
Carbon source Sucrose 5 mM
+1.3
+0.9
+0.7
+0.7
+0.6
pH
Minimal media pH7
+1.2
+0.9
+0.8
+0.6
+0.8
carbon source
D-Ribose (C)
+0.7
+0.5
+1.4
+0.7
+0.9
resistance
10% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine
+1.6
+0.7
+0.9
+0.4
+0.6
resistance
50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
+1.7
+0.7
+1.1
+0.5
+0.6
carbon source
Putrescine (C)
+1.1
+1.7
+1.0
+0.6
+0.5
carbon source
Uridine (C)
+0.6
+1.3
+1.4
+0.8
+1.0
carbon source
D-Ribose (C)
+1.5
+1.1
+1.2
+0.6
+1.0
resistance
10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
+1.9
+1.3
+1.2
+0.7
+1.0
resistance
50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
+1.8
+1.2
+1.3
+0.8
+1.0
carbon source
L-Asparagine (C)
+1.3
+1.9
+1.4
+0.8
+0.8
carbon source
acetate (C)
+1.7
+1.2
+1.3
+0.9
+1.3
carbon source
D-Xylose (C)
+1.4
+1.1
+1.7
+0.9
+1.3
carbon source
acetate (C)
+1.7
+1.2
+1.5
+1.0
+1.3
pH
Minimal media pH6
+1.6
+1.8
+1.3
+0.9
+1.1
nitrogen source
D-Glucosamine Hydrochloride (N)
+1.7
+1.7
+1.7
+1.3
+1.0
carbon source
D-Xylose (C)
+2.2
+1.4
+1.9
+1.0
+1.5
carbon source
D-Trehalose (C)
+2.3
+2.3
+2.3
+1.6
+1.6
carbon source
D-Trehalose (C)
+2.3
+2.4
+2.4
+1.6
+1.7
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