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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_03650 and AO356_03655 are separated by 94 nucleotides
AO356_03655 and AO356_03660 overlap by 4 nucleotides
AO356_03660 and AO356_03665 are separated by 199 nucleotides
AO356_03665 and AO356_03670 overlap by 4 nucleotides
AO356_03650: AO356_03650 - heme ABC transporter permease, at 814,688 to 815,443
_03650
AO356_03655: AO356_03655 - heme transporter, at 815,538 to 816,206
_03655
AO356_03660: AO356_03660 - cytochrome C biogenesis protein CcmA, at 816,203 to 816,838
_03660
AO356_03665: AO356_03665 - flagellar hook-length control protein FliK, at 817,038 to 818,609
_03665
AO356_03670: AO356_03670 - flagellar biosynthesis protein FlhB, at 818,606 to 818,935
_03670
Group
Condition
AO356
_03650
AO356
_03655
AO356
_03660
AO356
_03665
AO356
_03670
anaerobic
Nitrate reduction with yeast extract
N.D.
N.D.
N.D.
-1.9
+0.1
carbon source
Carbon source D-Mannitol 5 mM
N.D.
N.D.
N.D.
-1.0
-0.2
carbon source
acetate (C)
N.D.
N.D.
N.D.
-0.9
-0.2
carbon source
Carbon source Sucrose 10 mM
N.D.
N.D.
N.D.
-1.3
+0.1
stress
1-ethyl-3-methylimidazolium acetate 5 mM
N.D.
N.D.
N.D.
-0.7
-0.4
stress
KB-half, Pseudo5 supernatant
N.D.
N.D.
N.D.
-1.0
-0.0
carbon source
L-Arabinose (C)
N.D.
N.D.
N.D.
-0.6
-0.3
resistance
50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
N.D.
N.D.
N.D.
-0.7
-0.2
stress
nitrite 10 mM
N.D.
N.D.
N.D.
-0.4
-0.4
stress
Doxycycline hyclate 0.0001 mg/ml
N.D.
N.D.
N.D.
-0.7
-0.1
stress
Cisplatin 0.0032 mg/ml
N.D.
N.D.
N.D.
-0.7
-0.1
resistance
50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine
N.D.
N.D.
N.D.
-0.7
-0.0
motility
outer cut, LB soft agar motility assay
N.D.
N.D.
N.D.
-1.2
+0.4
lb
LB
N.D.
N.D.
N.D.
-0.4
-0.4
carbon source
Carbon source Sucrose 5 mM
N.D.
N.D.
N.D.
-0.7
-0.0
nitrogen source
D-Serine (N)
N.D.
N.D.
N.D.
-0.1
-0.7
motility
outer cut, LB soft agar motility assay
N.D.
N.D.
N.D.
-0.6
+0.3
stress
methylglyoxal 0.016 vol%
N.D.
N.D.
N.D.
-0.4
+0.3
stress
syringaldehyde 5 mM
N.D.
N.D.
N.D.
-0.4
+0.4
stress
acetate 40 mM
N.D.
N.D.
N.D.
+0.6
-0.6
survival
4C survival; 192 hrs
N.D.
N.D.
N.D.
-0.6
+0.6
stress
Choline chloride 300 mM
N.D.
N.D.
N.D.
+0.4
-0.3
stress
Chloramphenicol 0.005 mg/ml
N.D.
N.D.
N.D.
-0.3
+0.7
carbon source
L-Asparagine (C)
N.D.
N.D.
N.D.
+0.6
-0.2
carbon source
L-Proline (C)
N.D.
N.D.
N.D.
+0.7
-0.2
carbon source
Glycerol (C)
N.D.
N.D.
N.D.
+0.7
-0.0
nitrogen source
Putrescine (N)
N.D.
N.D.
N.D.
+0.7
-0.0
stress
Cholin acetate 20 mM
N.D.
N.D.
N.D.
+0.8
-0.1
carbon source
Glycerol (C)
N.D.
N.D.
N.D.
+0.8
+0.0
nitrogen source
Cytosine (N)
N.D.
N.D.
N.D.
+0.6
+0.4
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