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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_03590 and AO356_03595 overlap by 12 nucleotides
AO356_03595 and AO356_03600 are separated by 151 nucleotides
AO356_03600 and AO356_03605 are separated by 57 nucleotides
AO356_03605 and AO356_03610 are separated by 446 nucleotides
AO356_03590: AO356_03590 - hypothetical protein, at 804,750 to 804,977
_03590
AO356_03595: AO356_03595 - carbon storage regulator, at 804,966 to 805,166
_03595
AO356_03600: AO356_03600 - putative efflux pump, required for thallium (I) resistance
(from data)
, at 805,318 to 805,650
_03600
AO356_03605: AO356_03605 - short-chain dehydrogenase, at 805,708 to 806,523
_03605
AO356_03610: AO356_03610 - hypothetical protein, at 806,970 to 809,063
_03610
Group
Condition
AO356
_03590
AO356
_03595
AO356
_03600
AO356
_03605
AO356
_03610
stress
Sisomicin 0.001 mg/ml
N.D.
-1.1
-0.0
-1.0
-0.3
motility
outer cut, LB soft agar motility assay
N.D.
-2.7
+0.1
+0.2
+0.1
stress
Cholin acetate 40 mM
N.D.
-1.2
-0.4
-0.1
-0.1
carbon source
Uridine (C)
N.D.
-1.3
-0.1
-0.0
-0.0
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
N.D.
-1.2
+0.2
-0.2
-0.2
stress
Thallium(I) acetate 0.008 mg/ml
N.D.
-0.7
-1.5
+0.4
+0.4
survival
4C survival; 24 hrs
N.D.
-1.6
+0.2
+0.0
+0.0
resistance
10% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine
N.D.
-1.3
+0.0
-0.1
+0.0
carbon source
Carbon source Sucrose 2.5 mM
N.D.
-1.1
-0.0
-0.0
-0.1
stress
1-ethyl-3-methylimidazolium chloride 200 mM
N.D.
-1.0
-0.2
-0.1
-0.0
stress
perchlorate 75 mM
N.D.
-0.9
+0.1
-0.1
-0.4
resistance
50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
N.D.
-1.1
-0.1
-0.1
-0.0
carbon source
L-Lysine (C)
N.D.
-0.8
-0.3
-0.1
-0.1
carbon source
L-Proline (C)
N.D.
-1.0
-0.2
-0.0
+0.1
carbon source
L-Glutamine (C)
N.D.
-1.0
-0.1
+0.1
+0.1
carbon source
L-Glutamine (C)
N.D.
-0.8
-0.1
-0.0
+0.1
stress
Thallium(I) acetate 0.008 mg/ml
N.D.
-0.5
-1.1
+0.5
+0.4
pH
Minimal media pH6
N.D.
-0.8
+0.1
+0.2
-0.2
stress
Spectinomycin 0.025 mg/ml
N.D.
-0.9
+0.1
+0.1
+0.1
carbon source
Carbon source Sucrose 5 mM
N.D.
-0.7
+0.1
+0.1
-0.2
stress
Aluminum chloride 5 mM
N.D.
+0.5
+0.1
+0.0
-1.2
stress
Benzalkonium Chloride 0.008 mg/ml
N.D.
-0.6
+0.2
+0.4
-0.2
stress
Benzalkonium Chloride 0.016 mg/ml
N.D.
+0.7
-0.1
-0.2
-0.5
anaerobic
Nitrate reduction with yeast extract
N.D.
-0.5
+0.5
-0.0
+0.3
motility
outer cut, LB soft agar motility assay
N.D.
+0.2
+0.6
-0.4
-0.1
stress
copper (II) chloride 2.5 mM
N.D.
+0.7
-0.0
+0.1
-0.3
carbon source
Carbon source Sucrose 2.5 mM
N.D.
+0.5
-0.1
+0.3
-0.2
carbon source
Carbon source Sucrose 5 mM
N.D.
+0.9
-0.2
-0.0
-0.1
motility
outer cut, LB soft agar motility assay
N.D.
+0.5
+0.4
-0.2
+0.1
nitrogen source
L-Phenylalanine (N)
N.D.
+0.9
+0.1
-0.0
-0.0
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