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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_03360 and AO356_03365 are separated by 25 nucleotides
AO356_03365 and AO356_03370 are separated by 7 nucleotides
AO356_03370 and AO356_03375 are separated by 24 nucleotides
AO356_03375 and AO356_03380 are separated by 80 nucleotides
AO356_03360: AO356_03360 - hypothetical protein, at 747,643 to 748,884
_03360
AO356_03365: AO356_03365 - UDP-N-acetylglucosamine 2-epimerase, at 748,910 to 749,974
_03365
AO356_03370: AO356_03370 - glycosyl transferase, at 749,982 to 751,088
_03370
AO356_03375: AO356_03375 - ExsB family protein, at 751,113 to 752,267
_03375
AO356_03380: AO356_03380 - glycosyl amidation-associated protein WbuZ, at 752,348 to 753,109
_03380
Group
Condition
AO356
_03360
AO356
_03365
AO356
_03370
AO356
_03375
AO356
_03380
nitrogen source
D-Glucosamine Hydrochloride (N)
-5.4
-4.9
N.D.
-4.2
-4.5
nitrogen source
L-Phenylalanine (N)
-2.0
-2.8
N.D.
-3.9
-4.6
survival
4C survival; 192 hrs
-3.0
-3.9
N.D.
-3.4
-1.3
carbon source
NAG (C)
-3.6
-3.8
N.D.
-2.0
-1.3
motility
outer cut, LB soft agar motility assay
-3.1
-2.6
N.D.
-3.9
-1.0
carbon source
D-Trehalose (C)
-3.8
-4.1
N.D.
-1.6
-1.0
stress
perchlorate 75 mM
-2.5
-1.7
N.D.
-3.8
-2.4
stress
Cobalt chloride 0.08 mM
-3.2
-2.9
N.D.
-1.3
-2.6
pH
Minimal media pH9
-1.9
-3.3
N.D.
-3.0
-1.5
nitrogen source
Adenine (N)
-2.7
-3.0
N.D.
-1.8
-2.2
nitrogen source
L-Leucine (N)
-2.2
-2.0
N.D.
-2.9
-2.4
nitrogen source
Gly-Glu (N)
-2.4
-1.9
N.D.
-2.7
-2.4
stress
acetate 40 mM
-4.3
-2.8
N.D.
-1.2
-0.9
carbon source
D-Trehalose (C)
-3.5
-2.4
N.D.
-2.0
-1.0
stress
Cobalt chloride 0.08 mM
-2.6
-2.6
N.D.
-0.9
-2.7
stress
Choline chloride 500 mM
-2.1
-2.0
N.D.
-3.3
-1.2
nitrogen source
Urea (N)
-2.1
-2.0
N.D.
-2.3
-2.1
nitrogen source
Inosine (N)
-3.3
-1.7
N.D.
-2.8
-0.4
survival
4C survival; 360 hrs
-2.6
-2.1
N.D.
-3.2
-0.3
motility
outer cut, LB soft agar motility assay
-3.6
-1.7
N.D.
-1.1
-1.7
nitrogen source
Thymine (N)
-2.1
-1.3
N.D.
-0.8
-3.8
stress
Choline chloride 300 mM
-2.0
-2.1
N.D.
-1.9
-1.7
carbon source
L-Lysine (C)
-2.1
-1.2
N.D.
-3.1
-1.3
stress
nitrate 200 mM
-2.1
-1.8
N.D.
-2.8
-0.9
survival
4C survival; 1152 hrs
-1.5
-2.7
N.D.
-2.2
-1.0
stress
Benzethonium chloride 0.025 mM
-1.7
-2.6
N.D.
-0.8
-2.1
anaerobic
Nitrate reduction with yeast extract
+1.4
-0.8
N.D.
-4.0
-1.6
stress
Doxycycline hyclate 0.0001 mg/ml
+2.4
+2.0
N.D.
+3.0
+2.8
stress
Lomefloxacin 0.0005 mM
+2.3
+2.7
N.D.
+3.3
+2.6
phage
P5ss-5 phage 10-1 dilution SM calcl2
+7.1
+7.7
N.D.
+5.8
+0.7
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