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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_03265 and AO356_03270 are separated by 230 nucleotides
AO356_03270 and AO356_03275 are separated by 11 nucleotides
AO356_03275 and AO356_03280 are separated by 233 nucleotides
AO356_03280 and AO356_03285 are separated by 105 nucleotides
AO356_03265: AO356_03265 - chemotaxis protein, at 724,012 to 725,493
_03265
AO356_03270: AO356_03270 - terminase, at 725,724 to 726,098
_03270
AO356_03275: AO356_03275 - acyl-CoA thioesterase, at 726,110 to 726,907
_03275
AO356_03280: AO356_03280 - metal-chelation protein CHAD, at 727,141 to 727,902
_03280
AO356_03285: AO356_03285 - hypothetical protein, at 728,008 to 728,298
_03285
Group
Condition
AO356
_03265
AO356
_03270
AO356
_03275
AO356
_03280
AO356
_03285
stress
Lomefloxacin 0.0005 mM
-0.5
-1.7
-0.3
-0.1
-0.2
carbon source
L-Arginine (C)
-0.0
-0.4
-0.1
-0.2
-0.8
stress
Paraquat dichloride 0.0625 mg/ml
-0.1
-0.8
-0.4
-0.3
+0.1
phage
P5ss-5 phage 10-1 dilution SM calcl2
-0.1
-0.6
-0.1
-0.5
+0.1
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
-0.2
-0.6
-0.1
-0.8
+0.5
stress
1-ethyl-3-methylimidazolium chloride 200 mM
+0.3
-1.4
+0.3
-0.1
-0.1
motility
outer cut, LB soft agar motility assay
-0.1
-1.2
+0.3
-0.3
+0.5
nitrogen source
L-tyrosine (N)
-0.2
-0.6
-0.2
-0.3
+0.5
stress
Sisomicin 0.001 mg/ml
+0.4
-0.2
+0.1
+0.3
-1.3
stress
Cephalothin 2 mg/ml
+0.2
-1.2
+0.2
-0.1
+0.4
motility
outer cut, LB soft agar motility assay
-0.4
-0.7
-0.0
-0.0
+0.7
stress
Cholin acetate 40 mM
+0.3
-1.1
+0.1
-0.0
+0.5
lb
LB
-0.2
-0.7
-0.0
-0.1
+0.8
stress
KB-half, Pseudo5 supernatant
-0.3
-0.4
-0.2
-0.3
+1.0
motility
outer cut, LB soft agar motility assay
-0.2
-1.2
+0.4
-0.1
+1.2
stress
acetate 40 mM
+0.4
-1.1
+0.5
+0.0
+0.3
stress
1-ethyl-3-methylimidazolium acetate 20 mM
+0.4
-0.9
+0.2
+0.1
+0.5
resistance
50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
-0.4
+1.2
+0.2
-0.3
-0.3
survival
4C survival; 192 hrs
+0.1
-0.8
+0.2
+0.2
+0.7
anaerobic
Nitrate reduction with yeast extract
+0.2
-0.2
-0.8
+0.2
+1.0
carbon source
D-Xylose (C)
+0.1
-0.7
+0.1
+0.0
+1.1
pH
Minimal media pH6
+0.2
-0.4
+0.0
+0.2
+0.8
temperature
LB 20C
+0.2
-0.3
-0.2
+0.1
+1.1
motility
outer cut, LB soft agar motility assay
-0.4
-0.5
+0.6
+0.1
+1.4
stress
Vanillin 2.5 mM
+0.1
-0.3
+0.2
+0.2
+1.1
stress
methylglyoxal 0.016 vol%
-0.1
-0.2
+0.4
+0.3
+1.0
stress
Vanillin 1.25 mM
+0.1
-0.2
+0.1
+0.1
+1.4
survival
4C survival; 1152 hrs
+0.4
+0.2
+0.0
+0.2
+0.9
stress
copper (II) chloride 2.5 mM
+0.3
+0.4
-0.0
+0.7
+0.9
survival
4C survival; 672 hrs
+0.2
+0.9
+0.1
+0.0
+1.2
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