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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_03115 and AO356_03120 are separated by 142 nucleotides
AO356_03120 and AO356_03125 are separated by 127 nucleotides
AO356_03125 and AO356_03130 are separated by 64 nucleotides
AO356_03130 and AO356_03135 are separated by 61 nucleotides
AO356_03115: AO356_03115 - spermidine synthase, at 697,070 to 697,825
_03115
AO356_03120: AO356_03120 - phospho-2-dehydro-3-deoxyheptonate aldolase, at 697,968 to 699,314
_03120
AO356_03125: AO356_03125 - cytoplasmic protein, at 699,442 to 700,671
_03125
AO356_03130: AO356_03130 - hypothetical protein, at 700,736 to 700,954
_03130
AO356_03135: AO356_03135 - LysR family transcriptional regulator, at 701,016 to 701,906
_03135
Group
Condition
AO356
_03115
AO356
_03120
AO356
_03125
AO356
_03130
AO356
_03135
nitrogen source
Inosine (N)
+0.1
-0.1
-0.0
-1.5
-1.3
resistance
10% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine
+0.2
+0.1
+0.1
-1.4
-1.3
carbon source
NAG (C)
+0.1
-0.0
+0.0
-1.3
-0.9
resistance
20 mM putrescine
+0.1
+0.1
+0.1
-1.2
-1.1
carbon source
D-Trehalose (C)
+0.0
-0.0
-0.1
-0.8
-1.1
carbon source
D-Galactose (C)
+0.0
-0.1
-0.1
-0.8
-0.9
resistance
10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
+0.0
-0.1
+0.0
-0.9
-0.8
carbon source
D-Trehalose (C)
+0.1
+0.2
-0.1
-0.7
-1.3
resistance
50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
-0.1
+0.1
+0.2
-1.0
-0.9
nitrogen source
Adenine (N)
+0.1
-0.0
+0.0
-0.9
-0.8
pH
Minimal media pH6
-0.2
-0.0
-0.0
-0.5
-0.5
resistance
50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
+0.2
-0.0
+0.0
-0.7
-0.8
carbon source
D-Galactose (C)
+0.1
-0.1
-0.1
-0.5
-0.6
resistance
10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
+0.0
+0.2
+0.1
-0.7
-0.6
nitrogen source
Uridine (N)
+0.2
-0.0
+0.0
-0.6
-0.6
carbon source
D-Xylose (C)
+0.2
-0.1
-0.1
-0.3
-0.5
resistance
50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine
+0.0
+0.2
+0.0
-0.6
-0.6
nitrogen source
D-Serine (N)
+0.4
+0.1
+0.0
-0.7
-0.5
motility
outer cut, LB soft agar motility assay
+0.4
-0.3
+0.2
+0.6
-0.2
carbon source
Fumarate (C)
-0.2
-0.2
+0.0
+0.5
+0.5
anaerobic
Nitrate reduction with yeast extract
-0.2
+0.2
-0.1
+0.2
+0.5
carbon source
L-Histidine (C)
+0.1
-0.3
-0.0
+0.6
+0.5
nitrogen source
L-Leucine (N)
+0.4
-0.1
+0.1
+0.4
+0.2
stress
KB-half, Pseudo5 supernatant
+0.2
+0.4
+0.1
+0.2
+0.3
nitrogen source
L-tyrosine (N)
-0.3
+0.0
+0.1
+1.0
+0.6
carbon source
L-Histidine (C)
+0.2
-0.4
-0.0
+1.0
+0.6
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
-0.1
+0.8
+0.2
+0.5
+0.4
carbon source
L-Lysine (C)
+0.4
+0.1
-0.0
+0.8
+0.5
carbon source
L-Lysine (C)
+0.4
+0.0
+0.0
+1.1
+0.7
nitrogen source
Gly-Glu (N)
+0.0
-0.0
+0.1
+1.2
+1.3
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