Fitness for 5 genes in Pseudomonas fluorescens FW300-N2C3

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500 ntAO356_02600 and AO356_02605 are separated by 143 nucleotidesAO356_02605 and AO356_02610 are separated by 1145 nucleotidesAO356_02610 and AO356_02615 are separated by 96 nucleotidesAO356_02615 and AO356_02620 are separated by 270 nucleotides AO356_02600: AO356_02600 - nucleotide cyclase, at 570,966 to 573,350 _02600 AO356_02605: AO356_02605 - 50S rRNA methyltransferase, at 573,494 to 575,764 _02605 AO356_02610: AO356_02610 - dihydroorotate dehydrogenase (quinone), at 576,910 to 577,929 _02610 AO356_02615: AO356_02615 - hypothetical protein, at 578,026 to 579,177 _02615 AO356_02620: AO356_02620 - hypothetical protein, at 579,448 to 579,885 _02620
Group Condition AO356_02600 AO356_02605 AO356_02610 AO356_02615 AO356_02620
stress KB-half, Pseudo5 supernatant -0.0 -0.2 N.D. -0.4 -0.6
stress 1-ethyl-3-methylimidazolium chloride 200 mM -0.3 -0.0 N.D. -0.2 -0.5
carbon source L-Histidine (C) -0.3 -0.1 N.D. +0.0 -0.5
stress KB-half, Pseudo11 supernatant, 100 uM FeCl3 -0.1 -0.1 N.D. -0.4 -0.4
resistance 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine -0.2 +0.0 N.D. -0.0 -0.5
stress Bacitracin 2 mg/ml -0.0 -0.3 N.D. +0.0 -0.4
carbon source L-Lysine (C) +0.1 +0.1 N.D. -0.1 -0.7
resistance 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine -0.2 +0.1 N.D. +0.0 -0.5
stress Spectinomycin 0.025 mg/ml +0.2 -0.1 N.D. +0.1 -0.8
carbon source Ethanol (C) +0.1 -0.0 N.D. -0.1 -0.5
stress nitrite 10 mM -0.1 -0.0 N.D. +0.3 -0.6
stress Sisomicin 0.001 mg/ml -0.2 +0.1 N.D. +0.2 -0.5
resistance 50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine -0.2 +0.1 N.D. +0.1 -0.5
nitrogen source Urea (N) -0.2 +0.1 N.D. +0.1 -0.4
carbon source D-Galactose (C) +0.1 +0.0 N.D. +0.1 -0.5
resistance 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine -0.3 +0.2 N.D. +0.1 -0.3
carbon source L-Lysine (C) +0.1 +0.0 N.D. +0.1 -0.5
carbon source D,L-Lactate (C) +0.1 +0.1 N.D. +0.3 -0.7
phage P5ss-5 phage 10-1 dilution SM calcl2 -0.3 -0.2 N.D. +0.1 +0.3
stress Cobalt chloride 0.08 mM +0.2 +0.1 N.D. +0.2 -0.3
stress Cisplatin 0.0032 mg/ml +0.1 +0.3 N.D. +0.1 -0.3
stress Lomefloxacin 0.0005 mM +0.5 +0.0 N.D. -0.2 -0.1
pH Minimal media pH6 -0.2 +0.0 N.D. +0.2 +0.4
stress Paraquat dichloride 0.0625 mg/ml +0.1 +0.3 N.D. +0.3 -0.3
pH Minimal media pH8 -0.1 +0.0 N.D. +0.1 +0.5
stress Cobalt chloride 0.08 mM +0.2 +0.2 N.D. +0.3 -0.1
stress Paraquat dichloride 0.0625 mg/ml +0.2 +0.4 N.D. +0.1 -0.2
nitrogen source D-Serine (N) +0.1 +0.4 N.D. +0.0 +0.2
temperature LB 37C +0.1 +0.1 N.D. +0.2 +0.3
motility outer cut, LB soft agar motility assay -0.1 +0.0 N.D. +0.2 +0.6
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