Fitness for 5 genes in Pseudomonas fluorescens FW300-N2C3

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500 ntAO356_02325 and AO356_02330 are separated by 30 nucleotidesAO356_02330 and AO356_02335 are separated by 590 nucleotidesAO356_02335 and AO356_02340 are separated by 101 nucleotidesAO356_02340 and AO356_02345 are separated by 174 nucleotides AO356_02325: AO356_02325 - 3-ketoacyl-CoA thiolase, at 502,313 to 503,488 _02325 AO356_02330: AO356_02330 - multifunctional fatty acid oxidation complex subunit alpha, at 503,519 to 505,666 _02330 AO356_02335: AO356_02335 - hypothetical protein, at 506,257 to 506,694 _02335 AO356_02340: AO356_02340 - universal stress protein UspA, at 506,796 to 507,236 _02340 AO356_02345: AO356_02345 - ABC transporter ATP-binding protein, at 507,411 to 509,327 _02345
Group Condition AO356_02325 AO356_02330 AO356_02335 AO356_02340 AO356_02345
stress Sisomicin 0.001 mg/ml -4.2 N.D. -4.1 N.D. -0.3
carbon source L-Lysine (C) -1.8 N.D. -0.8 N.D. -1.1
carbon source Tween 20 (C) -3.5 N.D. -0.2 N.D. +0.1
carbon source D-Lactate (C) -1.3 N.D. -1.7 N.D. -0.2
carbon source D-Lactate (C) -2.3 N.D. -0.6 N.D. -0.2
survival 4C survival; 360 hrs -1.4 N.D. -1.6 N.D. +0.1
temperature LB 20C -1.4 N.D. -1.4 N.D. +0.1
stress Cholin acetate 40 mM -1.7 N.D. -0.7 N.D. -0.4
carbon source Tween 20 (C) -2.5 N.D. -0.1 N.D. -0.1
nitrogen source L-Leucine (N) -2.9 N.D. +0.2 N.D. +0.1
temperature LB 10C +0.1 N.D. -2.5 N.D. -0.1
temperature LB 10C -0.0 N.D. -2.3 N.D. -0.3
carbon source D-Alanine (C) -2.3 N.D. -0.1 N.D. -0.2
carbon source L-Leucine (C) -1.7 N.D. -0.3 N.D. -0.4
stress 1-ethyl-3-methylimidazolium acetate 20 mM -1.7 N.D. -0.5 N.D. -0.3
carbon source L-Lysine (C) -0.5 N.D. -1.6 N.D. -0.2
motility outer cut, LB soft agar motility assay -1.2 N.D. -0.6 N.D. -0.4
stress Thallium(I) acetate 0.008 mg/ml -1.5 N.D. -0.7 N.D. +0.0
carbon source L-Lactate (C) -1.7 N.D. -0.5 N.D. +0.1
carbon source Putrescine (C) -2.0 N.D. -0.1 N.D. +0.2
stress 1-ethyl-3-methylimidazolium acetate 10 mM -1.1 N.D. -0.8 N.D. +0.2
survival 4C survival; 192 hrs -1.1 N.D. -0.9 N.D. +0.5
carbon source acetate (C) +0.8 N.D. -1.8 N.D. -0.3
survival 4C survival; 1152 hrs +0.1 N.D. -1.7 N.D. +0.5
survival 4C survival; 672 hrs +0.7 N.D. -2.0 N.D. +0.1
stress Doxycycline hyclate 0.0001 mg/ml -1.6 N.D. +0.2 N.D. +0.5
stress Thallium(I) acetate 0.008 mg/ml +1.1 N.D. -0.8 N.D. -0.3
stress copper (II) chloride 2.5 mM +1.5 N.D. -1.9 N.D. +0.3
stress sodium fluoride 6.25 mM -0.8 N.D. +1.3 N.D. -0.1
anaerobic Nitrate reduction with yeast extract +0.8 N.D. -0.7 N.D. +0.8
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