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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_02275 and AO356_02280 are separated by 11 nucleotides
AO356_02280 and AO356_02285 are separated by 274 nucleotides
AO356_02285 and AO356_02290 are separated by 171 nucleotides
AO356_02290 and AO356_02295 are separated by 241 nucleotides
AO356_02275: AO356_02275 - hypothetical protein, at 491,298 to 491,864
_02275
AO356_02280: AO356_02280 - helicase, at 491,876 to 494,569
_02280
AO356_02285: AO356_02285 - beta-hexosaminidase, at 494,844 to 495,854
_02285
AO356_02290: AO356_02290 - TetR family transcriptional regulator, at 496,026 to 496,733
_02290
AO356_02295: AO356_02295 - LexA family transcriptional regulator, at 496,975 to 497,583
_02295
Group
Condition
AO356
_02275
AO356
_02280
AO356
_02285
AO356
_02290
AO356
_02295
survival
4C survival; 360 hrs
+0.2
-0.1
-2.3
-2.5
N.D.
survival
4C survival; 192 hrs
-0.4
+0.1
-2.2
-1.8
N.D.
pH
Minimal media pH6
-0.3
+0.1
-1.0
-2.6
N.D.
carbon source
Carbon source Sucrose 5 mM
-0.1
+0.0
-1.5
-2.0
N.D.
pH
Minimal media pH8
+0.1
+0.1
-2.1
-1.5
N.D.
carbon source
Carbon source Sucrose 2.5 mM
+0.0
+0.1
-2.1
-1.3
N.D.
carbon source
acetate (C)
-0.0
-0.1
-0.6
-2.6
N.D.
carbon source
Carbon source Sucrose 5 mM
+0.2
+0.1
-1.2
-2.4
N.D.
carbon source
Carbon source Sucrose 10 mM
-0.0
-0.0
-1.0
-1.9
N.D.
carbon source
acetate (C)
-0.0
-0.2
-0.8
-1.9
N.D.
pH
Minimal media pH9
-0.1
-0.0
-1.9
-0.9
N.D.
stress
nitrate 200 mM
-0.9
+0.0
-0.5
-1.6
N.D.
carbon source
Carbon source Sucrose 10 mM
+0.1
+0.1
-1.4
-1.7
N.D.
carbon source
L-Proline (C)
-0.2
+0.0
-0.1
-2.5
N.D.
carbon source
Carbon source Sucrose 2.5 mM
+0.1
+0.1
-1.9
-0.8
N.D.
pH
Minimal media pH6
-0.2
+0.1
-1.2
-1.2
N.D.
nitrogen source
Gly-Glu (N)
+0.3
+0.0
-0.3
-2.4
N.D.
nitrogen source
L-Leucine (N)
+0.3
-0.1
-0.1
-2.2
N.D.
carbon source
D-Lactate (C)
+0.3
+0.1
+0.0
-2.4
N.D.
anaerobic
Nitrate reduction with yeast extract
+0.8
-0.3
-1.6
-0.7
N.D.
carbon source
D-Fructose (C)
+0.1
+0.1
+0.4
-2.2
N.D.
stress
Cholin acetate 40 mM
+0.6
-0.1
-0.5
-1.6
N.D.
motility
outer cut, LB soft agar motility assay
-0.2
-0.1
-1.7
+0.6
N.D.
stress
Sisomicin 0.001 mg/ml
+0.3
+0.1
+0.5
-2.0
N.D.
stress
1-ethyl-3-methylimidazolium lysine 10 mM
+0.5
+0.0
+0.6
-1.8
N.D.
survival
4C survival; 1152 hrs
+0.5
-0.0
-2.0
+1.7
N.D.
carbon source
L-Histidine (C)
+0.2
-0.0
-0.8
+2.2
N.D.
carbon source
L-Histidine (C)
+0.2
+0.0
-0.7
+2.3
N.D.
stress
Choline chloride 500 mM
+1.9
+0.3
-0.2
+0.5
N.D.
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
+0.4
-0.1
+0.6
+2.1
N.D.
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