Fitness for 5 genes in Pseudomonas fluorescens FW300-N2C3

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500 ntAO356_02190 and AO356_02195 are separated by 201 nucleotidesAO356_02195 and AO356_02200 are separated by 109 nucleotidesAO356_02200 and AO356_02205 are separated by 184 nucleotidesAO356_02205 and AO356_02210 are separated by 61 nucleotides AO356_02190: AO356_02190 - hypothetical protein, at 471,421 to 472,110 _02190 AO356_02195: AO356_02195 - phosphatase, at 472,312 to 472,932 _02195 AO356_02200: AO356_02200 - chromosome partitioning protein ParA, at 473,042 to 473,305 _02200 AO356_02205: AO356_02205 - NADPH-dependent 7-cyano-7-deazaguanine reductase, at 473,490 to 474,320 _02205 AO356_02210: AO356_02210 - copper-binding protein, at 474,382 to 474,903 _02210
Group Condition AO356_02190 AO356_02195 AO356_02200 AO356_02205 AO356_02210
stress Chlorite 0.08 mM -1.8 -0.0 N.D. -3.2 -1.1
stress Sisomicin 0.001 mg/ml -2.5 +0.3 N.D. -2.3 -1.1
carbon source Carbon source Sucrose 5 mM -2.1 +0.3 N.D. -2.9 -0.2
stress nitrite 10 mM -0.6 -0.1 N.D. -1.7 -2.3
carbon source Carbon source Sucrose 2.5 mM -2.6 -0.6 N.D. -1.8 +0.4
motility outer cut, LB soft agar motility assay -1.2 +0.6 N.D. -1.9 -2.0
stress Benzalkonium Chloride 0.016 mg/ml -4.0 -0.1 N.D. -0.7 +0.2
carbon source acetate (C) -1.2 -0.4 N.D. -2.6 -0.2
stress Benzethonium chloride 0.025 mM -4.1 +0.2 N.D. -0.8 +0.2
stress Rifampicin 0.0024 mg/ml -2.9 -0.0 N.D. -2.2 +0.6
carbon source D-Lactate (C) -2.7 -0.0 N.D. -1.4 -0.3
stress Bacitracin 2 mg/ml -3.4 -0.3 N.D. -0.2 -0.6
survival 4C survival; 360 hrs -2.6 -0.3 N.D. -0.9 -0.7
nitrogen source D-Alanine (N) -2.3 +0.0 N.D. -2.2 +0.2
carbon source D-Lactate (C) -2.6 -0.3 N.D. -0.7 -0.6
pH Minimal media pH9 -3.0 +0.0 N.D. -0.7 -0.2
stress Benzalkonium Chloride 0.008 mg/ml -4.3 +0.4 N.D. +0.1 -0.1
stress Nalidixic 0.004 mg/ml -4.3 +0.1 N.D. +0.7 +0.2
stress syringaldehyde 10 mM -3.4 +0.3 N.D. -0.3 +0.2
carbon source NAG (C) -2.1 -0.5 N.D. -1.1 +0.6
stress Chlorite 0.04 mM -1.3 +0.4 N.D. -2.1 -0.1
resistance 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine -2.6 +0.3 N.D. -1.1 +0.4
stress Spectinomycin 0.025 mg/ml -2.7 +0.4 N.D. -1.0 +0.2
carbon source Carbon source D-Mannitol 10 mM -2.5 +0.6 N.D. -1.2 +0.1
resistance 10% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine -1.3 +0.4 N.D. -2.0 +0.3
carbon source Inosine (C) -3.2 -0.1 N.D. +0.4 +0.5
stress Doxycycline hyclate 0.0001 mg/ml -2.1 +0.2 N.D. +1.4 -0.7
stress Lomefloxacin 0.0005 mM -2.6 +0.2 N.D. +1.6 +0.5
stress 1-ethyl-3-methylimidazolium chloride 200 mM -1.2 +0.2 N.D. +2.3 -0.3
stress copper (II) chloride 2.5 mM -1.3 -0.5 N.D. +2.7 +2.3
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