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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_00510 and AO356_00515 are separated by 826 nucleotides
AO356_00515 and AO356_00520 are separated by 11 nucleotides
AO356_00520 and AO356_00525 overlap by 4 nucleotides
AO356_00525 and AO356_00530 are separated by 77 nucleotides
AO356_00510: AO356_00510 - cobalamin biosynthesis protein CobW, at 107,903 to 108,967
_00510
AO356_00515: AO356_00515 - cobalt transporter, at 109,794 to 109,982
_00515
AO356_00520: AO356_00520 - cobalt transporter, at 109,994 to 110,689
_00520
AO356_00525: AO356_00525 - cobalamin biosynthesis protein CobE, at 110,686 to 111,090
_00525
AO356_00530: AO356_00530 - precorrin-4 C11-methyltransferase, at 111,168 to 111,914
_00530
Group
Condition
AO356
_00510
AO356
_00515
AO356
_00520
AO356
_00525
AO356
_00530
nitrogen source
Adenosine (N)
N.D.
N.D.
-1.0
-1.5
-2.5
motility
outer cut, LB soft agar motility assay
N.D.
N.D.
-0.9
-1.2
-1.0
carbon source
Tween 20 (C)
N.D.
N.D.
-0.8
-1.0
-1.2
carbon source
D-Lactate (C)
N.D.
N.D.
-0.3
-1.6
-1.0
pH
Minimal media pH8
N.D.
N.D.
-0.8
-1.3
-0.8
carbon source
D-Sorbitol (C)
N.D.
N.D.
-0.1
-1.4
-1.2
anaerobic
Nitrate reduction with yeast extract
N.D.
N.D.
-1.3
-0.2
-1.0
nitrogen source
nitrite (N)
N.D.
N.D.
-0.6
-1.3
-0.6
nitrogen source
Ammonium chloride (N)
N.D.
N.D.
-0.8
-0.7
-1.0
carbon source
L-Asparagine (C)
N.D.
N.D.
-1.1
-0.5
-0.7
nitrogen source
L-Leucine (N)
N.D.
N.D.
-2.3
+0.1
-0.2
pH
Minimal media pH7
N.D.
N.D.
-0.9
-0.6
-0.8
stress
syringaldehyde 5 mM
N.D.
N.D.
-0.6
-0.9
-0.7
carbon source
L-Malic (C)
N.D.
N.D.
+0.2
-1.1
-1.2
carbon source
m-Inositol (C)
N.D.
N.D.
-0.3
-0.3
-1.5
pH
Minimal media pH8
N.D.
N.D.
-1.2
-0.3
-0.5
nitrogen source
D-Alanine (N)
N.D.
N.D.
-0.0
-0.8
-1.1
carbon source
L-Proline (C)
N.D.
N.D.
-0.1
-1.0
-0.7
carbon source
Tween 20 (C)
N.D.
N.D.
-0.7
-0.3
-0.7
carbon source
L-Glutamic (C)
N.D.
N.D.
+0.0
-0.3
-1.4
carbon source
L-Malic (C)
N.D.
N.D.
+0.2
-0.7
-1.1
motility
outer cut, LB soft agar motility assay
N.D.
N.D.
+0.5
-0.8
-0.7
carbon source
Carbon source Sucrose 10 mM
N.D.
N.D.
+0.4
-0.6
-0.8
carbon source
casaminos (C)
N.D.
N.D.
-0.8
+0.5
-0.6
carbon source
Carbon source D,L-Lactate 10 mM
N.D.
N.D.
+0.2
-1.4
+0.4
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
N.D.
N.D.
-0.5
+0.6
-0.6
carbon source
Carbon source D-Glucose 10 mM
N.D.
N.D.
+0.4
-1.2
+0.4
stress
Sisomicin 0.001 mg/ml
N.D.
N.D.
-0.2
+1.0
-0.8
stress
Nickel (II) chloride 0.625 mM
N.D.
N.D.
+0.3
-0.8
+0.6
nitrogen source
D-Serine (N)
N.D.
N.D.
-0.8
+0.7
+0.4
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