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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_00320 and AO356_00325 are separated by 145 nucleotides
AO356_00325 and AO356_00330 are separated by 1 nucleotides
AO356_00330 and AO356_00335 are separated by 1339 nucleotides
AO356_00335 and AO356_00340 are separated by 222 nucleotides
AO356_00320: AO356_00320 - hypothetical protein, at 59,499 to 60,803
_00320
AO356_00325: AO356_00325 - RNA helicase, at 60,949 to 63,414
_00325
AO356_00330: AO356_00330 - hypothetical protein, at 63,416 to 64,216
_00330
AO356_00335: AO356_00335 - integrase, at 65,556 to 67,397
_00335
AO356_00340: AO356_00340 - tRNA-Gly, at 67,620 to 67,695
_00340
Group
Condition
AO356
_00320
AO356
_00325
AO356
_00330
AO356
_00335
AO356
_00340
stress
KB-half, Pseudo5 supernatant
-0.3
-0.1
-0.0
-0.1
N.D.
carbon source
Carbon source D-Mannitol 2.5 mM
+0.0
+0.1
-0.6
-0.1
N.D.
stress
Nickel (II) chloride 0.625 mM
-0.2
-0.1
+0.1
-0.1
N.D.
stress
Nalidixic 0.004 mg/ml
+0.0
+0.1
-0.2
-0.2
N.D.
carbon source
D-Ribose (C)
-0.3
-0.1
+0.3
-0.1
N.D.
carbon source
Ethanol (C)
+0.1
-0.0
-0.3
+0.1
N.D.
motility
outer cut, LB soft agar motility assay
+0.4
-0.1
-0.3
-0.1
N.D.
stress
Bacitracin 2 mg/ml
+0.1
+0.1
-0.2
-0.1
N.D.
carbon source
Inosine (C)
-0.2
-0.1
+0.2
+0.0
N.D.
anaerobic
Nitrate reduction with yeast extract
+0.3
-0.1
-0.2
-0.0
N.D.
carbon source
D-Xylose (C)
-0.1
-0.0
+0.2
-0.1
N.D.
carbon source
L-Lysine (C)
+0.1
-0.0
-0.2
+0.2
N.D.
carbon source
Carbon source Sucrose 5 mM
+0.1
+0.1
-0.2
+0.1
N.D.
motility
outer cut, LB soft agar motility assay
-0.0
+0.1
+0.2
-0.2
N.D.
resistance
50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
+0.1
-0.1
+0.3
-0.1
N.D.
stress
perchlorate 75 mM
+0.3
+0.1
-0.1
+0.0
N.D.
stress
Cobalt chloride 0.08 mM
-0.1
+0.1
+0.3
+0.1
N.D.
motility
outer cut, LB soft agar motility assay
+0.4
-0.0
-0.2
+0.1
N.D.
survival
4C survival; 672 hrs
-0.1
+0.0
+0.3
+0.1
N.D.
carbon source
Fumarate (C)
-0.1
+0.0
+0.3
+0.2
N.D.
stress
Cholin acetate 40 mM
-0.1
+0.1
+0.3
+0.1
N.D.
pH
Minimal media pH8
+0.2
+0.2
+0.1
-0.1
N.D.
temperature
LB 37C
+0.1
+0.1
+0.2
+0.1
N.D.
stress
Vanillin 1.25 mM
+0.3
+0.1
+0.2
-0.0
N.D.
pH
Minimal media pH7
+0.1
+0.1
+0.4
+0.1
N.D.
stress
Cisplatin 0.0032 mg/ml
-0.0
+0.5
+0.4
-0.1
N.D.
stress
1-ethyl-3-methylimidazolium chloride 200 mM
+0.2
+0.2
+0.4
+0.1
N.D.
stress
copper (II) chloride 2.5 mM
+0.2
+0.2
+0.2
+0.4
N.D.
stress
Cisplatin 0.0032 mg/ml
+0.1
+0.5
+0.6
+0.1
N.D.
stress
Lomefloxacin 0.0005 mM
+0.2
+0.8
+0.5
+0.1
N.D.
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