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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
AO353_29030 and AO353_29035 are separated by 518 nucleotides
AO353_29035 and AO353_29040 are separated by 44 nucleotides
AO353_29040 and AO353_29045 are separated by 231 nucleotides
AO353_29045 and AO353_29050 are separated by 548 nucleotides
AO353_29030: AO353_29030 - GntR family transcriptional regulator, at 6,309,207 to 6,309,917
_29030
AO353_29035: AO353_29035 - MFS transporter, at 6,310,436 to 6,311,788
_29035
AO353_29040: AO353_29040 - glucarate dehydratase, at 6,311,833 to 6,313,182
_29040
AO353_29045: AO353_29045 - glycosyltransferase, at 6,313,414 to 6,314,268
_29045
AO353_29050: AO353_29050 - 2'-5' RNA ligase, at 6,314,817 to 6,315,362
_29050
Group
Condition
AO353
_29030
AO353
_29035
AO353
_29040
AO353
_29045
AO353
_29050
motility
outer cut, LB soft agar motility assay
+0.0
-0.2
-0.7
-0.2
-0.5
anaerobic
Anaerobic, acetate donor; nitrate and iron
-0.0
-0.2
-0.2
-0.3
-0.6
carbon source
D-Glucose
-0.2
-0.0
-0.3
-0.2
-0.4
carbon source
acetate (C)
+0.4
-0.2
-0.4
-0.1
-0.7
pH
Minimal media pH9
-0.3
-0.2
+0.1
+0.1
-0.6
stress
Cobalt chloride 0.32 mM
+0.3
+0.1
-0.7
+0.0
-0.4
stress
Sisomicin 0.008 mg/ml
-0.2
-0.4
+0.6
+0.1
-0.7
carbon source
L-Phenylalanine (C)
+0.2
-0.2
-0.2
-0.1
-0.3
nitrogen source
Glycine (N)
+0.1
+0.1
+0.1
-0.2
-0.6
motility
outer cut, LB soft agar motility assay
+0.4
+0.0
-0.1
-0.5
-0.3
carbon source
Trisodium citrate (C)
+0.2
-0.2
-0.3
-0.2
-0.1
carbon source
L-Valine (C)
-0.3
+0.1
-0.1
+0.2
-0.3
stress
Thallium(I) acetate 0.008 mg/ml
-0.3
+0.1
-0.1
+0.4
-0.3
stress
Cisplatin 0.006 mg/ml
-0.0
+0.0
-0.5
+0.5
-0.3
stress
methylglyoxal 0.009 vol%
+0.2
-0.1
-0.6
+0.1
+0.1
anaerobic
N1B4wt with N2E3m denitrifying replicate C
+0.2
-0.1
+0.1
+0.2
-0.6
carbon source
pyruvate (C)
-0.0
+0.2
-0.4
+0.2
-0.2
carbon source
Ethanol (C)
-0.0
+0.0
+0.1
-0.6
+0.4
carbon source
L-Asparagine (C)
-0.1
+0.0
+0.1
-0.4
+0.3
stress
sodium fluoride 6.25 mM
+0.5
+0.1
-0.2
-0.2
-0.2
stress
Thallium(I) acetate 0.016 mg/ml
-0.1
-0.1
+0.2
+0.4
-0.4
carbon source
L-Alanine (C)
+0.0
-0.3
-0.2
+0.1
+0.4
carbon source
Fumarate (C)
+0.1
-0.1
-0.3
-0.0
+0.4
stress
Thallium(I) acetate 0.016 mg/ml
-0.2
+0.2
+0.0
+0.3
-0.3
stress
Cisplatin 0.006 mg/ml
+0.0
-0.1
+0.2
+0.4
-0.3
lb
LB
-0.1
+0.2
+0.2
+0.3
-0.5
stress
Thallium(I) acetate 0.008 mg/ml
-0.3
+0.1
+0.2
+0.5
-0.2
carbon source
L-Citrulline
-0.2
+0.2
+0.3
+0.4
-0.3
pH
Minimal media pH9
-0.2
-0.1
+0.2
+0.0
+0.5
motility
outer cut, LB soft agar motility assay
+0.1
+0.0
-0.2
+0.2
+0.5
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