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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_27920 and AO353_27925 are separated by 153 nucleotides
AO353_27925 and AO353_27930 are separated by 957 nucleotides
AO353_27930 and AO353_27935 are separated by 148 nucleotides
AO353_27935 and AO353_27940 are separated by 35 nucleotides
AO353_27920: AO353_27920 - arginine N-succinyltransferase, at 6,091,461 to 6,092,495
_27920
AO353_27925: AO353_27925 - hypothetical protein, at 6,092,649 to 6,092,981
_27925
AO353_27930: AO353_27930 - LysR family transcriptional regulator, at 6,093,939 to 6,094,823
_27930
AO353_27935: AO353_27935 - succinyl-CoA--3-ketoacid-CoA transferase, at 6,094,972 to 6,095,670
_27935
AO353_27940: AO353_27940 - succinyl-CoA--3-ketoacid-CoA transferase, at 6,095,706 to 6,096,365
_27940
Group
Condition
AO353
_27920
AO353
_27925
AO353
_27930
AO353
_27935
AO353
_27940
carbon source
Carnitine Hydrochloride (C)
+0.1
+0.6
-2.5
-2.5
-2.1
motility
outer cut, LB soft agar motility assay
-1.6
-3.8
+0.7
-0.3
-0.0
carbon source
L-Phenylalanine (C)
+0.2
-0.2
-1.5
-0.5
-1.6
stress
Paraquat dichloride 0.032 mg/mL
-0.3
-0.7
-1.0
-0.4
+0.0
nitrogen source
Carnitine Hydrochloride (N)
-0.3
+0.1
-0.8
-0.5
-0.6
carbon source
L-Leucine (C)
-0.3
+0.9
+0.4
-1.3
-1.2
motility
outer cut, LB soft agar motility assay
-0.3
-0.5
+1.0
-1.4
-0.2
nitrogen source
Glycine (N)
-0.2
-0.7
-0.2
-0.6
+0.4
stress
Thallium(I) acetate 0.008 mg/ml
-0.5
-0.9
+0.4
-0.2
-0.1
stress
Doxycycline hyclate 0.0006 mg/ml
+0.3
-1.4
-0.5
+0.1
+0.2
carbon source
D-Ribose (C)
-0.3
-1.5
+0.1
+0.2
+0.2
stress
1-ethyl-3-methylimidazolium lysine 10.5 mM
+0.4
-1.1
-0.3
-0.4
+0.1
stress
copper (II) chloride 2.5 mM
-0.2
+0.1
-0.3
-1.1
+0.2
stress
Cisplatin 0.006 mg/ml
-0.8
+1.1
-0.6
-0.9
+0.0
stress
Lomefloxacin 0.001 mM
-0.2
-0.1
-0.1
+0.8
-1.2
carbon source
L-Citrulline
-0.5
-0.8
-0.5
+0.7
+0.4
nitrogen source
L-Arginine (N)
-0.9
+0.1
+0.4
-0.4
+0.1
carbon source
acetate (C)
+0.1
-0.9
+0.5
-0.4
+0.1
pH
Minimal media pH9
+0.1
+0.6
-0.8
-0.2
-0.2
stress
methylglyoxal 0.008 vol%
+0.3
-0.7
+0.5
-0.5
+0.2
stress
Sisomicin 0.008 mg/ml
-0.1
-1.0
+0.4
+0.3
+0.3
anaerobic
Anaerobic, acetate donor; nitrate and iron
-0.4
+0.4
-0.3
-0.4
+0.7
nitrogen source
L-Phenylalanine (N)
-0.2
-0.9
+0.3
+0.7
+0.4
carbon source
Tween 20 (C)
+0.2
-0.8
+0.1
+0.6
+0.3
stress
Nalidixic 0.004 mg/ml
-0.5
+0.6
-0.3
+0.4
+0.3
motility
outer cut, LB soft agar motility assay
-0.9
+1.4
-0.2
+0.9
-0.2
survival
4C survival; 360 hrs
-0.9
+0.1
+0.3
+1.0
+0.8
carbon source
L-Ornithine
+0.4
-0.3
+0.7
+0.2
+0.6
carbon source
L-Isoleucine (C)
-0.2
+0.7
+0.6
+0.8
-0.2
survival
4C survival; 672 hrs
-1.6
+0.2
+2.1
+3.5
+2.6
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