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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_27020 and AO353_27025 are separated by 185 nucleotides
AO353_27025 and AO353_27030 are separated by 431 nucleotides
AO353_27030 and AO353_27035 are separated by 0 nucleotides
AO353_27035 and AO353_27040 are separated by 3 nucleotides
AO353_27020: AO353_27020 - transcriptional regulator, at 5,921,068 to 5,921,526
_27020
AO353_27025: AO353_27025 - 2-nitropropane dioxygenase, at 5,921,712 to 5,922,776
_27025
AO353_27030: AO353_27030 - molybdate ABC transporter substrate-binding protein, at 5,923,208 to 5,923,975
_27030
AO353_27035: AO353_27035 - molybdenum ABC transporter permease, at 5,923,976 to 5,924,656
_27035
AO353_27040: AO353_27040 - molybdenum ABC transporter ATP-binding protein, at 5,924,660 to 5,925,748
_27040
Group
Condition
AO353
_27020
AO353
_27025
AO353
_27030
AO353
_27035
AO353
_27040
anaerobic
N1B4wt with N2E3m denitrifying replicate C
+0.3
-0.0
-2.2
-2.3
-1.9
denitrifying
Nitrate reduction, replicate C
+0.4
-0.7
-1.2
-0.8
-1.3
denitrifying
Nitrate reduction, replicate B
+0.1
-0.3
-1.3
-0.9
-1.2
carbon source
L-Citrulline
-0.1
-0.4
-0.1
-1.1
-0.2
carbon source
L-Citrulline
-0.2
-0.5
-0.4
-0.2
-0.4
temperature
LB 20C
-0.3
-0.5
-0.2
-0.0
-0.5
stress
Lomefloxacin 0.001 mM
-0.3
+0.2
-0.5
-0.3
-0.5
carbon source
L-Alanine (C)
+0.2
+0.0
+0.1
-1.0
-0.6
anaerobic
Anaerobic, acetate donor; nitrate and iron
-0.2
-0.4
-0.5
+0.5
-0.7
stress
methylglyoxal 0.009 vol%
-0.1
-0.0
-0.6
-0.6
+0.3
carbon source
L-Citrulline
-0.0
-0.5
+0.2
-0.3
-0.4
stress
Doxycycline hyclate 0.0006 mg/ml
+0.2
+0.2
-0.8
-0.2
-0.5
nitrogen source
Urea (N)
-0.1
+0.4
-0.4
-0.3
-0.4
stress
Ceftazidime 0.12 mg/ml
-0.4
+0.5
-0.3
-0.3
-0.2
carbon source
D-Alanine (C)
+0.1
+0.5
+0.0
-0.7
-0.4
nitrogen source
Inosine (N)
-0.0
+0.6
-0.5
-0.1
-0.4
carbon source
acetate (C)
-0.3
+0.5
+0.0
-0.5
-0.1
nitrogen source
Parabanic Acid (N)
-0.1
+0.4
+0.1
-0.5
-0.2
stress
acetate 68 mM
-0.6
+0.4
-0.3
+0.1
+0.1
stress
Cisplatin 0.006 mg/ml
+0.4
-1.0
+0.1
+0.4
-0.2
stress
Cisplatin 0.006 mg/ml
-0.4
-0.4
-0.2
+0.3
+0.4
motility
outer cut, LB soft agar motility assay
-0.1
+0.8
-0.3
+0.1
-0.7
nitrogen source
Thymine (N)
-0.1
+0.7
-0.2
-0.1
-0.3
stress
Thallium(I) acetate 0.008 mg/ml
-0.1
+0.0
-0.3
+0.7
-0.4
stress
Doxycycline hyclate 0.0002 mg/ml
-0.2
+0.8
-0.2
-0.2
-0.0
carbon source
m-Inositol (C)
+0.4
-0.4
+0.3
-0.2
+0.2
carbon source
L-Phenylalanine (C)
+0.4
+0.8
-0.1
-1.2
+0.3
pH
Minimal media pH9
-0.2
+0.1
+1.0
+0.7
+0.4
pH
Minimal media pH9
+0.2
+0.1
+0.6
+0.7
+0.5
stress
Chlorite 0.16 mM
+0.1
+0.2
+0.6
+0.8
+0.7
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