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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_26785 and AO353_26790 are separated by 176 nucleotides
AO353_26790 and AO353_26795 are separated by 166 nucleotides
AO353_26795 and AO353_26800 overlap by 23 nucleotides
AO353_26800 and AO353_26805 overlap by 8 nucleotides
AO353_26785: AO353_26785 - hypothetical protein, at 5,868,022 to 5,868,387
_26785
AO353_26790: AO353_26790 - Glutaminase (EC 3.5.1.2)
(from data)
, at 5,868,564 to 5,869,472
_26790
AO353_26795: AO353_26795 - NAD-dependent deacetylase, at 5,869,639 to 5,870,502
_26795
AO353_26800: AO353_26800 - hydrogen cyanide synthase, at 5,870,480 to 5,871,739
_26800
AO353_26805: AO353_26805 - (2Fe-2S)-binding protein, at 5,871,732 to 5,873,141
_26805
Group
Condition
AO353
_26785
AO353
_26790
AO353
_26795
AO353
_26800
AO353
_26805
carbon source
L-Glutamine (C)
-0.2
-3.3
-0.4
-0.2
+0.1
carbon source
L-Glutamine (C)
-0.1
-3.4
+0.0
+0.2
+0.3
carbon source
L-Serine (C)
-0.9
-0.5
-0.0
+0.1
+0.1
denitrifying
Nitrate reduction, replicate B
-0.4
-0.6
+0.7
-0.7
-0.0
pH
Minimal media pH9
+0.5
+0.1
-1.7
-0.1
+0.3
anaerobic
N1B4wt with N2E3m denitrifying replicate C
+0.1
-0.0
-0.7
+0.3
-0.6
nitrogen source
Parabanic Acid (N)
+0.3
-0.8
-0.0
-0.0
-0.3
stress
methylglyoxal 0.009 vol%
+0.7
-0.5
-0.8
-0.6
+0.3
nitrogen source
L-Serine (N)
+0.2
+0.1
-0.7
-0.3
-0.1
stress
Cisplatin 0.006 mg/ml
-0.1
+0.2
-0.6
+0.1
-0.4
carbon source
L-Citrulline
-0.6
+0.2
-0.8
+0.1
+0.2
carbon source
L-Citrulline
+0.3
+0.4
-1.8
-0.1
+0.4
stress
Thallium(I) acetate 0.008 mg/ml
+0.3
+0.0
-0.9
+0.1
-0.1
pH
Minimal media pH9
+0.1
-0.4
-0.3
-0.3
+0.3
carbon source
propionate (C)
+0.4
-0.4
-0.3
-0.3
+0.1
carbon source
L-Citrulline
-0.8
-0.2
+0.4
+0.0
-0.0
carbon source
acetate (C)
-0.2
+0.2
-0.7
+0.3
-0.1
denitrifying
Nitrate reduction, replicate C
-0.6
+0.0
+0.5
-0.1
-0.2
carbon source
L-Arginine (C)
-0.3
-0.2
+0.3
+0.3
-0.4
stress
perchlorate 75 mM
-0.2
+0.2
-0.4
-0.2
+0.5
stress
Doxycycline hyclate 0.0006 mg/ml
+0.3
-0.3
+0.3
+0.2
-0.5
motility
outer cut, LB soft agar motility assay
+0.5
-0.2
+0.5
-0.2
-0.4
carbon source
L-Citrulline
-0.5
-0.0
+0.5
-0.3
+0.6
carbon source
L-Ornithine
-0.7
+0.5
+0.4
+0.2
+0.4
nitrogen source
D-Serine (N)
+0.3
-0.1
+0.6
+0.3
-0.3
stress
nitrate 200 mM
-0.2
+0.2
+0.7
-0.1
+0.1
motility
outer cut, LB soft agar motility assay
+0.4
-0.3
+0.7
-0.1
+0.2
stress
Chlorite 0.16 mM
-0.2
+0.3
+0.6
-0.0
+0.4
stress
benzoic 0.58 mg/ml
+0.3
+0.3
+0.6
+0.0
+0.1
stress
Cisplatin 0.006 mg/ml
+0.5
+0.5
+0.3
+0.5
+0.1
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