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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_26710 and AO353_26715 are separated by 25 nucleotides
AO353_26715 and AO353_26720 are separated by 14 nucleotides
AO353_26720 and AO353_26725 are separated by 195 nucleotides
AO353_26725 and AO353_26730 are separated by 79 nucleotides
AO353_26710: AO353_26710 - citrate-proton symporter, at 5,846,082 to 5,847,386
_26710
AO353_26715: AO353_26715 - molybdenum ABC transporter substrate-binding protein, at 5,847,412 to 5,848,188
_26715
AO353_26720: AO353_26720 - LysR family transcriptional regulator, at 5,848,203 to 5,849,120
_26720
AO353_26725: AO353_26725 - potassium transporter KefB, at 5,849,316 to 5,851,124
_26725
AO353_26730: AO353_26730 - DNA alkylation repair protein, at 5,851,204 to 5,852,307
_26730
Group
Condition
AO353
_26710
AO353
_26715
AO353
_26720
AO353
_26725
AO353
_26730
carbon source
L-Isoleucine (C)
-0.7
-0.2
-0.2
-0.0
-0.1
motility
outer cut, LB soft agar motility assay
-0.0
-0.1
-0.3
+0.1
-0.5
carbon source
L-Leucine (C)
+0.1
-0.5
+0.1
-0.1
-0.5
stress
Vancomycin 1 mg/ml
-0.5
-0.2
-0.2
+0.1
-0.0
carbon source
L-Citrulline
-0.4
-0.2
+0.2
-0.4
+0.1
stress
Thallium(I) acetate 0.008 mg/ml
-0.2
-0.4
+0.1
+0.1
-0.2
stress
1-ethyl-3-methylimidazolium lysine 10.5 mM
-0.3
+0.2
+0.1
-0.4
-0.0
carbon source
L-Citrulline
-0.0
+0.1
-0.5
-0.2
+0.2
stress
Cisplatin 0.006 mg/ml
-0.6
-0.2
+0.3
+0.1
-0.0
pH
Minimal media pH9
-0.2
-0.3
+0.2
-0.2
+0.1
carbon source
L-Serine (C)
-0.5
+0.2
-0.1
+0.1
+0.1
stress
Cisplatin 0.006 mg/ml
-0.1
-0.2
+0.2
+0.1
-0.4
anaerobic
Anaerobic, acetate donor; nitrate and iron
-0.2
-0.0
-0.2
-0.3
+0.4
anaerobic
N1B4wt with N2E3m denitrifying replicate C
-0.3
+0.3
-0.2
-0.2
+0.2
stress
1-ethyl-3-methylimidazolium acetate 30 mM
+0.1
+0.1
+0.0
-0.6
+0.2
nitrogen source
L-Phenylalanine (N)
+0.2
+0.2
-0.2
-0.1
-0.2
denitrifying
Nitrate reduction, replicate B
-0.3
-0.3
-0.0
+0.5
+0.1
survival
4C survival; 672 hrs
+0.4
-0.5
+0.4
+0.0
-0.0
motility
outer cut, LB soft agar motility assay
+0.3
+0.4
-0.0
+0.1
-0.3
stress
Thallium(I) acetate 0.008 mg/ml
+0.1
-0.2
+0.3
+0.4
-0.0
carbon source
L-Ornithine
+0.7
-0.5
+0.0
+0.3
+0.1
stress
1-ethyl-3-methylimidazolium acetate 20 mM
+0.0
+0.3
+0.2
-0.2
+0.3
stress
Doxycycline hyclate 0.0006 mg/ml
+0.2
+0.3
+0.0
+0.5
-0.3
stress
Nalidixic 0.004 mg/ml
+0.3
+0.2
+0.0
+0.3
+0.2
carbon source
L-Citrulline
-0.0
-0.0
+0.4
+0.0
+0.7
denitrifying
Nitrate reduction, replicate C
+0.1
+0.4
+0.2
+0.4
-0.1
carbon source
D-Glucose
+0.1
+0.4
-0.1
+0.4
+0.3
carbon source
acetate (C)
+0.2
+0.7
+0.3
+0.1
-0.3
stress
1-ethyl-3-methylimidazolium chloride 170 mM
+0.4
+0.5
+0.0
+0.1
+0.2
stress
Doxycycline hyclate 0.0002 mg/ml
+0.2
+0.9
+0.1
+0.1
+0.2
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