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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_26620 and AO353_26625 are separated by 103 nucleotides
AO353_26625 and AO353_26630 are separated by 195 nucleotides
AO353_26630 and AO353_26635 are separated by 187 nucleotides
AO353_26635 and AO353_26640 are separated by 1 nucleotides
AO353_26620: AO353_26620 - ArsR family transcriptional regulator, at 5,829,363 to 5,829,698
_26620
AO353_26625: AO353_26625 - MBL fold metallo-hydrolase, at 5,829,802 to 5,830,665
_26625
AO353_26630: AO353_26630 - ArsR family transcriptional regulator, at 5,830,861 to 5,831,349
_26630
AO353_26635: AO353_26635 - branched-chain alpha-ketoacid dehydrogenase, E1 component alpha subunit (EC 1.2.4.4)
(from data)
, at 5,831,537 to 5,832,772
_26635
AO353_26640: AO353_26640 - 2-oxoisovalerate dehydrogenase, at 5,832,774 to 5,833,832
_26640
Group
Condition
AO353
_26620
AO353
_26625
AO353
_26630
AO353
_26635
AO353
_26640
carbon source
L-Valine (C)
+0.6
+0.0
-3.4
-3.9
-3.9
carbon source
L-Isoleucine (C)
+0.5
-0.1
-3.1
-3.1
-3.9
carbon source
L-Leucine (C)
+0.3
+0.1
-3.2
-3.1
-3.5
stress
Sisomicin 0.008 mg/ml
-3.6
-0.8
+0.0
+0.1
-0.1
carbon source
acetate (C)
-0.9
-0.0
-0.3
-0.9
-1.6
motility
outer cut, LB soft agar motility assay
+0.2
-0.1
-0.7
-0.6
-1.1
carbon source
propionate (C)
+0.0
+0.0
-0.7
-0.8
-0.9
stress
methylglyoxal 0.009 vol%
-0.8
-0.4
+0.2
-0.5
-0.6
stress
Cisplatin 0.006 mg/ml
+0.3
+0.4
-1.5
-0.2
-1.1
carbon source
L-Serine (C)
-1.0
+0.1
-0.5
-0.4
-0.1
stress
Cobalt chloride 0.32 mM
-0.2
+0.2
-0.5
-0.5
-0.6
pH
Minimal media pH9
-0.5
-0.5
-0.3
-0.3
+0.4
survival
4C survival; 672 hrs
+0.7
+0.0
-0.5
-0.7
-0.5
carbon source
D-Trehalose (C)
-1.2
+0.1
+0.2
-0.3
+0.5
nitrogen source
Parabanic Acid (N)
+0.3
-0.4
+0.2
+0.1
-0.9
stress
Cisplatin 0.006 mg/ml
+0.6
+0.4
-0.7
-0.1
-0.8
carbon source
L-Aspartic Acid (C)
-0.4
+0.7
+0.1
-0.3
-0.4
motility
outer cut, LB soft agar motility assay
+0.5
+0.9
-0.6
-0.4
-0.4
stress
Lomefloxacin 0.001 mM
-0.4
+0.4
+0.5
+0.5
+0.5
carbon source
L-Citrulline
-0.5
+0.1
+0.5
+0.7
+0.7
anaerobic
Anaerobic, acetate donor; nitrate and iron
+0.4
-0.2
-0.1
+0.6
+0.9
carbon source
L-Ornithine
-0.4
-0.5
+0.7
+1.1
+0.7
carbon source
L-Ornithine
-0.2
+0.0
+0.3
+0.7
+1.0
carbon source
L-Citrulline
-0.4
-0.1
+0.3
+0.7
+1.3
carbon source
D-Glucose
-0.2
+0.5
+0.5
+0.7
+0.6
carbon source
L-Ornithine
+0.1
-0.6
+0.7
+1.1
+0.9
carbon source
L-Ornithine
+0.2
-0.3
+0.7
+0.5
+1.0
carbon source
D-Glucose
-0.1
-0.2
+0.7
+1.0
+0.8
carbon source
L-Citrulline
-0.2
-0.4
+1.2
+0.9
+0.9
carbon source
L-Citrulline
-0.9
+0.5
+0.7
+1.3
+0.8
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