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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_25880 and AO353_25885 are separated by 129 nucleotides
AO353_25885 and AO353_25890 are separated by 10 nucleotides
AO353_25890 and AO353_25895 are separated by 35 nucleotides
AO353_25895 and AO353_25900 are separated by 15 nucleotides
AO353_25880: AO353_25880 - sugar ABC transporter substrate-binding protein, at 5,707,204 to 5,708,514
_25880
AO353_25885: AO353_25885 - sugar ABC transporter permease, at 5,708,644 to 5,709,570
_25885
AO353_25890: AO353_25890 - sugar ABC transporter permease, at 5,709,581 to 5,710,411
_25890
AO353_25895: AO353_25895 - ABC transporter ATP-binding protein, at 5,710,447 to 5,711,550
_25895
AO353_25900: AO353_25900 - mannitol dehydrogenase, at 5,711,566 to 5,713,041
_25900
Group
Condition
AO353
_25880
AO353
_25885
AO353
_25890
AO353
_25895
AO353
_25900
carbon source
D-Mannose (C)
-3.1
-2.2
-2.2
-3.6
-2.4
carbon source
acetate (C)
-0.1
-1.1
-0.1
-0.4
-0.6
carbon source
D-Alanine (C)
+0.1
-1.3
+0.0
-0.4
-0.2
nitrogen source
Glycine (N)
-0.1
-0.5
+0.1
-0.6
-0.4
nitrogen source
L-Histidine (N)
-0.2
-0.7
-0.3
-0.1
-0.2
stress
Tetracycline 0.0016 mg/ml
-0.1
-0.5
-0.1
-0.4
-0.2
carbon source
L-Isoleucine (C)
+0.1
-0.1
-0.6
-0.1
-0.6
carbon source
L-Alanine (C)
+0.1
-0.9
-0.4
-0.1
+0.1
carbon source
L-Valine (C)
+0.1
-0.8
-0.4
-0.2
+0.2
carbon source
L-Leucine (C)
+0.2
-0.6
-0.0
-0.2
-0.4
pH
Minimal media pH9
+0.0
-0.4
-0.2
+0.3
-0.4
denitrifying
Nitrate reduction, replicate C
-0.1
-0.1
+0.4
-0.0
-0.8
anaerobic
Anaerobic, acetate donor; nitrate and iron
-0.3
-0.2
-0.4
+0.5
-0.2
stress
Cisplatin 0.006 mg/ml
+0.1
-0.3
-0.3
-0.3
+0.3
pH
Minimal media pH9
-0.1
+0.7
-0.4
-0.1
-0.5
stress
Sisomicin 0.008 mg/ml
-0.0
-0.7
+0.0
+0.5
-0.2
carbon source
Tween 20 (C)
-0.1
-0.7
-0.1
+0.5
+0.0
carbon source
D-Serine (C)
+0.1
-0.9
+0.2
+0.1
+0.1
motility
outer cut, LB soft agar motility assay
+0.1
+0.3
-0.7
-0.4
+0.4
stress
Doxycycline hyclate 0.0006 mg/ml
+0.3
-0.7
+0.2
+0.3
-0.3
carbon source
D-Glucose
-0.1
+0.3
-0.2
-0.6
+0.6
stress
perchlorate 75 mM
+0.2
-0.6
+0.3
+0.3
-0.1
motility
outer cut, LB soft agar motility assay
-0.1
-0.3
-0.5
+0.6
+0.5
stress
methylglyoxal 0.009 vol%
-0.2
+0.8
-0.7
+0.3
+0.0
carbon source
L-Ornithine
-0.1
-0.6
+0.0
+0.6
+1.0
carbon source
D-Glucose
-0.2
+0.2
-0.3
+0.3
+0.9
survival
4C survival; 360 hrs
-0.0
+0.8
-0.2
+0.4
+0.1
carbon source
L-Ornithine
-0.1
-0.5
-0.1
+0.8
+1.2
carbon source
D-Fructose (C)
+0.0
+0.0
-0.2
+0.0
+1.9
carbon source
D-Fructose (C)
+0.1
-0.0
+0.2
+0.1
+1.4
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