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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_25695 and AO353_25700 are separated by 83 nucleotides
AO353_25700 and AO353_25705 overlap by 20 nucleotides
AO353_25705 and AO353_25710 are separated by 249 nucleotides
AO353_25710 and AO353_25715 are separated by 8 nucleotides
AO353_25695: AO353_25695 - AMP-binding protein, at 5,665,982 to 5,667,646
_25695
AO353_25700: AO353_25700 - hypothetical protein, at 5,667,730 to 5,667,948
_25700
AO353_25705: AO353_25705 - AraC family transcriptional regulator, at 5,667,929 to 5,668,948
_25705
AO353_25710: AO353_25710 - iron transporter, at 5,669,198 to 5,670,049
_25710
AO353_25715: AO353_25715 - multidrug DMT transporter permease, at 5,670,058 to 5,671,257
_25715
Group
Condition
AO353
_25695
AO353
_25700
AO353
_25705
AO353
_25710
AO353
_25715
carbon source
L-Isoleucine (C)
-2.6
+0.3
-2.8
-0.5
-0.3
carbon source
L-Valine (C)
-1.8
+0.1
-1.7
-0.4
+0.1
stress
Sisomicin 0.008 mg/ml
-0.1
-0.9
-0.6
-0.4
-0.3
carbon source
butyrate (C)
-2.5
+0.8
-0.2
+0.2
-0.2
stress
methylglyoxal 0.009 vol%
-0.1
-0.9
-0.2
-0.5
-0.4
stress
Doxycycline hyclate 0.0006 mg/ml
+0.4
-2.1
-0.0
+0.6
-0.2
stress
1-ethyl-3-methylimidazolium acetate 20 mM
-0.1
-0.7
+0.1
-0.6
+0.1
denitrifying
Nitrate reduction, replicate C
-0.3
-0.5
-0.2
+0.1
-0.2
carbon source
Tween 20 (C)
+0.0
-0.9
-0.0
-0.4
+0.2
carbon source
D-Gluconic Acid (C)
+0.1
-1.0
+0.0
-0.2
+0.1
stress
Cisplatin 0.006 mg/ml
+0.3
-0.7
+0.1
-1.0
+0.5
stress
Paraquat dichloride 0.032 mg/mL
+0.0
-1.1
+0.3
-0.1
+0.1
carbon source
L-Leucine (C)
+0.4
+0.1
+0.2
-0.9
-0.4
carbon source
L-Citrulline
+0.0
-1.4
+0.3
+0.4
+0.1
nitrogen source
L-Arginine (N)
-0.4
+0.5
-0.0
-0.4
-0.1
survival
4C survival; 672 hrs
-0.1
+0.5
-0.3
-0.8
+0.4
carbon source
L-Citrulline
+0.2
-0.8
+0.5
-0.0
-0.4
nitrogen source
L-Glutamine (N)
-0.2
+0.5
+0.0
-0.4
-0.1
stress
perchlorate 75 mM
-0.3
+0.6
-0.1
-0.2
-0.2
motility
outer cut, LB soft agar motility assay
-0.0
-0.6
+0.0
+0.7
-0.1
stress
methylglyoxal 0.008 vol%
-0.1
+0.4
+0.2
-0.7
+0.2
carbon source
Ethanol (C)
+0.2
+0.5
+0.0
-0.7
+0.1
carbon source
L-Citrulline
-0.3
-0.2
+0.5
+0.1
+0.3
carbon source
L-Ornithine
+0.2
-0.6
+0.4
+0.3
+0.3
stress
Thallium(I) acetate 0.008 mg/ml
-0.0
+0.8
+0.2
-0.6
+0.2
nitrogen source
Parabanic Acid (N)
+0.2
+0.8
+0.2
-0.2
-0.2
carbon source
L-Proline (C)
+0.2
+1.1
-0.0
-0.4
+0.0
carbon source
L-Ornithine
+0.2
+0.7
+0.4
-0.2
-0.1
carbon source
L-Citrulline
-0.3
+0.7
+0.7
-0.0
+0.1
carbon source
L-Ornithine
+0.3
-0.1
+0.7
+0.9
+0.1
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