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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_25205 and AO353_25210 are separated by 44 nucleotides
AO353_25210 and AO353_25215 are separated by 30 nucleotides
AO353_25215 and AO353_25220 are separated by 79 nucleotides
AO353_25220 and AO353_25225 are separated by 57 nucleotides
AO353_25205: AO353_25205 - permease DsdX, at 5,561,016 to 5,562,359
_25205
AO353_25210: AO353_25210 - D-serine ammonia-lyase (EC 4.3.1.18)
(from data)
, at 5,562,404 to 5,563,756
_25210
AO353_25215: AO353_25215 - transcriptional regulator, at 5,563,787 to 5,564,722
_25215
AO353_25220: AO353_25220 - hypothetical protein, at 5,564,802 to 5,565,071
_25220
AO353_25225: AO353_25225 - hypothetical protein, at 5,565,129 to 5,565,398
_25225
Group
Condition
AO353
_25205
AO353
_25210
AO353
_25215
AO353
_25220
AO353
_25225
carbon source
D-Serine (C)
-2.7
-3.7
-4.0
-0.2
-0.1
nitrogen source
D-Serine (N)
-0.3
-1.9
-1.2
-0.2
-0.2
carbon source
L-Serine (C)
-0.4
-0.6
-0.6
+0.3
-0.1
carbon source
L-Citrulline
-0.1
-0.6
+0.4
-0.4
-0.6
stress
acetate 68 mM
-0.4
-0.1
-0.1
-0.7
-0.1
nitrogen source
nitrite (N)
-0.1
-0.0
-0.1
-0.9
-0.2
nitrogen source
L-Glutamine (N)
-0.1
+0.1
+0.1
-1.0
-0.4
carbon source
L-Alanine (C)
-0.2
+0.2
+0.1
-1.1
-0.2
carbon source
acetate (C)
-0.0
-0.3
+0.1
-1.1
+0.3
stress
Doxycycline hyclate 0.0006 mg/ml
-0.1
+0.1
+0.1
-0.8
-0.2
stress
copper (II) chloride 2.5 mM
-0.1
+0.2
+0.2
-0.8
-0.1
stress
Ceftazidime 0.12 mg/ml
-0.2
-0.2
+0.3
-0.7
+0.2
stress
Lomefloxacin 0.001 mM
-0.1
+0.1
-0.9
+0.5
-0.1
stress
Nalidixic 0.004 mg/ml
-0.3
+0.2
+0.2
-0.4
-0.2
motility
outer cut, LB soft agar motility assay
-0.3
-0.1
+0.4
-0.5
+0.4
motility
outer cut, LB soft agar motility assay
-0.3
+0.3
-0.5
+0.3
+0.0
stress
methylglyoxal 0.009 vol%
-0.2
+0.3
+0.3
-0.4
+0.1
motility
outer cut, LB soft agar motility assay
+0.2
+0.1
-0.4
-0.4
+0.5
carbon source
L-Aspartic Acid (C)
-0.0
+0.0
+0.2
+0.6
-0.5
anaerobic
Anaerobic, acetate donor; nitrate and iron
+0.1
+0.2
-0.8
+0.6
+0.4
stress
Chlorite 0.16 mM
-0.1
+0.1
-0.4
+0.7
+0.2
stress
Benzethonium chloride 0.025 mM
+0.0
+0.1
-0.4
+0.7
+0.3
stress
Furfuryl Alcohol 0.25 vol%
-0.3
+0.0
+0.3
+0.5
+0.2
carbon source
L-Ornithine
+0.1
+0.0
+0.2
+0.8
-0.3
stress
Polymyxin B sulfate 4 mg/ml
-0.1
-0.2
+0.1
+0.8
+0.2
stress
Rifampicin 0.005 mg/ml
+0.1
-0.2
+0.3
+0.4
+0.4
carbon source
L-Citrulline
+0.1
+0.0
+0.4
+0.7
-0.1
carbon source
Tween 20 (C)
-0.1
+0.3
+0.4
+0.7
+0.0
stress
perchlorate 75 mM
+0.2
+0.2
+0.3
+0.4
+0.3
carbon source
L-Glutamine (C)
+0.1
+0.1
+0.2
+1.0
+0.1
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