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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_24760 and AO353_24765 are separated by 108 nucleotides
AO353_24765 and AO353_24770 are separated by 36 nucleotides
AO353_24770 and AO353_24775 overlap by 4 nucleotides
AO353_24775 and AO353_24780 are separated by 80 nucleotides
AO353_24760: AO353_24760 - glutamine amidotransferase, at 5,458,241 to 5,459,038
_24760
AO353_24765: AO353_24765 - GCN5 family acetyltransferase, at 5,459,147 to 5,459,644
_24765
AO353_24770: AO353_24770 - FAD-dependent oxidoreductase, at 5,459,681 to 5,460,991
_24770
AO353_24775: AO353_24775 - ABC transporter substrate-binding protein, at 5,460,988 to 5,462,076
_24775
AO353_24780: AO353_24780 - glutamine synthetase, at 5,462,157 to 5,463,515
_24780
Group
Condition
AO353
_24760
AO353
_24765
AO353
_24770
AO353
_24775
AO353
_24780
nitrogen source
Parabanic Acid (N)
-0.4
-0.4
-0.6
-0.4
-0.3
carbon source
L-Proline (C)
-0.4
-0.6
-0.0
-0.7
+0.1
carbon source
L-Citrulline
-0.6
+0.1
-0.3
-0.3
-0.3
stress
Chlorite 0.16 mM
-0.7
-0.6
-0.0
+0.1
-0.3
denitrifying
Nitrate reduction, replicate B
-1.3
+0.2
+0.4
-0.3
-0.4
carbon source
propionate (C)
-0.7
-0.2
-0.0
-0.5
+0.3
stress
Paraquat dichloride 0.032 mg/mL
-1.4
+0.1
+0.2
-0.1
+0.1
carbon source
pyruvate (C)
-0.9
+0.3
+0.0
-0.3
-0.2
stress
copper (II) chloride 2.5 mM
-0.7
-0.4
+0.2
-0.3
+0.2
carbon source
acetate (C)
-1.2
+0.3
+0.6
-0.2
-0.5
stress
nitrate 200 mM
-0.5
+0.2
+0.3
-0.4
+0.1
pH
Minimal media pH9
+0.5
-1.0
-0.1
+0.1
+0.2
survival
4C survival; 360 hrs
+0.3
+0.0
-0.6
-0.4
+0.5
stress
Cobalt chloride 0.16 mM
-0.4
+0.4
+0.3
-0.2
-0.2
carbon source
L-Tryptophan (C)
+0.8
-0.9
-0.1
-0.1
+0.2
stress
1-ethyl-3-methylimidazolium acetate 30 mM
-0.8
+0.2
+0.2
+0.3
+0.1
motility
outer cut, LB soft agar motility assay
+0.5
+0.4
-0.2
-0.4
-0.3
radiation
Ionizing radiation, 126.196 Gy,
-0.3
+0.4
-0.1
-0.3
+0.4
carbon source
D-Glucose
+0.7
+0.0
-0.5
+0.3
-0.2
nitrogen source
L-Arginine (N)
+0.7
-0.5
+0.2
-0.1
+0.1
stress
Thallium(I) acetate 0.008 mg/ml
+0.6
-0.5
+0.4
+0.0
+0.1
motility
outer cut, LB soft agar motility assay
+0.1
-0.7
+0.2
+0.6
+0.4
carbon source
L-Citrulline
+0.2
+1.1
-0.3
-0.1
+0.0
stress
Cisplatin 0.006 mg/ml
+0.4
+0.2
+1.0
-0.7
+0.1
stress
Cholin acetate 38 mM
+0.7
-0.2
-0.1
+0.2
+0.4
stress
Benzalkonium Chloride 0.012 mg/ml
+1.0
-0.1
+0.3
-0.3
+0.1
stress
Chloramphenicol 0.0025 mg/ml
+0.5
+0.2
+0.2
-0.3
+0.5
stress
Thallium(I) acetate 0.016 mg/ml
+0.9
+0.2
+0.5
-0.2
-0.0
stress
Cisplatin 0.006 mg/ml
+0.4
+0.5
+0.6
+0.0
+0.1
stress
Doxycycline hyclate 0.0006 mg/ml
+0.9
-0.1
+0.1
+0.9
+0.0
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