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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_23965 and AO353_23970 are separated by 370 nucleotides
AO353_23970 and AO353_23975 are separated by 54 nucleotides
AO353_23975 and AO353_23980 overlap by 13 nucleotides
AO353_23980 and AO353_23985 are separated by 390 nucleotides
AO353_23965: AO353_23965 - RND transporter MFP subunit, at 5,262,225 to 5,263,478
_23965
AO353_23970: AO353_23970 - hypothetical protein, at 5,263,849 to 5,265,135
_23970
AO353_23975: AO353_23975 - diguanylate cyclase, at 5,265,190 to 5,268,210
_23975
AO353_23980: AO353_23980 - sugar ABC transporter substrate-binding protein, at 5,268,198 to 5,269,217
_23980
AO353_23985: AO353_23985 - isochorismate-pyruvate lyase, at 5,269,608 to 5,269,931
_23985
Group
Condition
AO353
_23965
AO353
_23970
AO353
_23975
AO353
_23980
AO353
_23985
carbon source
L-Ornithine
-1.1
-0.2
-0.2
-0.4
-0.0
stress
Cisplatin 0.006 mg/ml
-0.7
-0.1
-0.6
-0.0
-0.3
carbon source
L-Citrulline
-1.0
-0.6
-0.2
+0.1
+0.4
carbon source
L-Proline (C)
-0.5
-0.2
-0.2
-0.4
+0.1
stress
Doxycycline hyclate 0.0002 mg/ml
-0.7
+0.0
-0.1
-0.7
+0.3
carbon source
L-Citrulline
-0.7
+0.2
-0.2
+0.1
-0.6
motility
outer cut, LB soft agar motility assay
-0.6
+0.2
-0.0
-0.3
-0.3
stress
Doxycycline hyclate 0.0006 mg/ml
-0.2
-0.1
-0.1
-0.9
+0.3
carbon source
L-Ornithine
-0.6
-0.1
-0.0
-0.3
+0.3
pH
Minimal media pH9
-0.7
-0.2
+0.0
-0.2
+0.2
stress
Cholin acetate 19 mM
-0.4
-0.1
-0.2
-0.4
+0.4
denitrifying
Nitrate reduction, replicate C
-0.1
+0.3
+0.3
+0.0
-1.3
stress
Fusidic 0.125 mg/ml
-0.4
-0.2
+0.2
+0.4
-0.5
carbon source
Tween 20 (C)
-0.6
+0.1
+0.1
-0.3
+0.3
stress
Paraquat dichloride 0.032 mg/mL
+0.4
+0.1
-0.1
+0.0
-0.7
carbon source
L-Leucine (C)
-0.3
+0.0
-0.4
-0.2
+0.7
carbon source
L-Valine (C)
+0.3
+0.1
-0.3
+0.2
-0.5
stress
Furfuryl Alcohol 0.0625 vol%
-0.0
+0.1
-0.0
+0.5
-0.6
carbon source
L-Asparagine (C)
-0.5
+0.2
+0.1
-0.2
+0.3
carbon source
Ethanol (C)
-0.1
+0.2
+0.0
-0.6
+0.4
stress
Benzalkonium Chloride 0.012 mg/ml
-0.5
+0.2
-0.2
+0.1
+0.4
carbon source
L-Malic (C)
-0.2
-0.0
+0.1
-0.4
+0.6
carbon source
L-Serine (C)
-0.3
+0.2
-0.1
-0.2
+0.5
carbon source
m-Inositol (C)
-0.2
+0.1
-0.0
-0.2
+0.7
carbon source
acetate (C)
-0.2
-0.3
+0.3
-0.2
+0.8
stress
methylglyoxal 0.009 vol%
+1.1
-0.2
-0.1
+0.0
-0.1
carbon source
D-Glucose
+0.5
+0.0
-0.2
+0.4
+0.5
stress
Nickel (II) chloride 0.625 mM
+1.5
-0.1
+0.1
+0.0
+0.5
stress
Nickel (II) chloride 0.625 mM
+1.8
-0.1
-0.1
+0.2
+0.4
survival
4C survival; 672 hrs
-0.2
+0.1
+2.0
+1.8
+0.2
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