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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_23425 and AO353_23430 are separated by 281 nucleotides
AO353_23430 and AO353_23435 are separated by 2 nucleotides
AO353_23435 and AO353_23440 are separated by 1 nucleotides
AO353_23440 and AO353_23445 are separated by 36 nucleotides
AO353_23425: AO353_23425 - 3-demethylubiquinone-9 3-methyltransferase, at 5,131,462 to 5,131,965
_23425
AO353_23430: AO353_23430 - (2Fe-2S)-binding protein, at 5,132,247 to 5,132,708
_23430
AO353_23435: AO353_23435 - twin-arginine translocation pathway signal protein, at 5,132,711 to 5,134,879
_23435
AO353_23440: AO353_23440 - hypothetical protein, at 5,134,881 to 5,135,459
_23440
AO353_23445: AO353_23445 - glyoxalase, at 5,135,496 to 5,135,921
_23445
Group
Condition
AO353
_23425
AO353
_23430
AO353
_23435
AO353
_23440
AO353
_23445
motility
outer cut, LB soft agar motility assay
-2.1
-0.6
+0.3
-2.8
N.D.
stress
Furfuryl Alcohol 0.0625 vol%
-0.7
-0.7
+0.1
-0.6
N.D.
stress
Choline chloride 358 mM
-0.5
-0.1
+0.1
-1.1
N.D.
carbon source
propionate (C)
-0.7
-0.3
-0.1
-0.4
N.D.
motility
outer cut, LB soft agar motility assay
-0.6
-1.2
+0.0
+0.3
N.D.
carbon source
L-Phenylalanine (C)
+0.2
-0.4
-0.1
-1.0
N.D.
stress
acetate 68 mM
+0.3
-0.8
+0.0
-0.9
N.D.
carbon source
D-Glucose
-0.4
-0.2
+0.4
-1.1
N.D.
carbon source
L-Citrulline
-1.2
+0.2
+0.1
-0.3
N.D.
stress
Cobalt chloride 0.16 mM
-0.4
-0.5
+0.2
-0.5
N.D.
nitrogen source
Parabanic Acid (N)
-0.6
+0.4
-0.4
-0.5
N.D.
carbon source
L-Ornithine
-0.8
+0.1
+0.4
-0.6
N.D.
stress
1-ethyl-3-methylimidazolium acetate 30 mM
+0.3
-0.7
-0.0
-0.5
N.D.
carbon source
L-Citrulline
-0.8
+0.5
+0.1
-0.6
N.D.
carbon source
L-Valine (C)
-1.3
+0.1
+0.3
+0.2
N.D.
stress
1-ethyl-3-methylimidazolium acetate 15 mM
+0.3
-0.4
+0.1
-0.8
N.D.
stress
Cholin acetate 19 mM
+0.0
+0.6
-0.2
-0.8
N.D.
carbon source
L-Citrulline
-0.9
-0.1
+0.3
+0.3
N.D.
stress
Chloramphenicol 0.0025 mg/ml
-0.6
-0.4
+0.1
+0.6
N.D.
carbon source
L-Arginine (C)
-0.5
-0.4
+0.1
+0.7
N.D.
carbon source
L-Proline (C)
-0.5
-0.5
+0.0
+0.8
N.D.
stress
Thallium(I) acetate 0.016 mg/ml
+0.5
-0.8
+0.0
+0.2
N.D.
carbon source
m-Inositol (C)
-0.1
-0.6
-0.2
+0.9
N.D.
stress
Lomefloxacin 0.001 mM
+0.0
-0.7
-0.1
+0.9
N.D.
carbon source
L-Citrulline
-1.0
+0.3
+0.5
+0.5
N.D.
stress
Nalidixic 0.004 mg/ml
+0.3
+0.4
+0.3
-0.6
N.D.
survival
4C survival; 192 hrs
-0.7
+0.2
+0.2
+0.7
N.D.
carbon source
L-Glutamine (C)
+0.0
+0.7
+0.3
-0.5
N.D.
carbon source
L-Alanine (C)
+0.2
-0.7
+0.1
+0.8
N.D.
carbon source
L-Ornithine
-0.7
+0.4
+0.2
+0.9
N.D.
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