Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E3

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500 ntAO353_22875 and AO353_22880 are separated by 271 nucleotidesAO353_22880 and AO353_22885 are separated by 235 nucleotidesAO353_22885 and AO353_22890 are separated by 179 nucleotidesAO353_22890 and AO353_22895 overlap by 4 nucleotides AO353_22875: AO353_22875 - cell envelope protein, at 5,014,355 to 5,015,761 _22875 AO353_22880: AO353_22880 - polyketide cyclase, at 5,016,033 to 5,016,476 _22880 AO353_22885: AO353_22885 - peroxidase, at 5,016,712 to 5,017,212 _22885 AO353_22890: AO353_22890 - secretion protein HlyD, at 5,017,392 to 5,018,714 _22890 AO353_22895: AO353_22895 - multidrug transporter, at 5,018,711 to 5,021,815 _22895
Group Condition AO353_22875 AO353_22880 AO353_22885 AO353_22890 AO353_22895
survival 4C survival; 672 hrs -0.3 -0.1 N.D. -0.9 -0.7
motility outer cut, LB soft agar motility assay -0.7 -0.3 N.D. -0.3 -0.7
survival 4C survival; 360 hrs -0.1 -0.2 N.D. -0.8 -0.6
stress Rifampicin 0.005 mg/ml +0.2 -0.1 N.D. -1.2 -0.5
carbon source L-Leucine (C) -0.2 -0.6 N.D. -0.3 -0.5
carbon source m-Inositol (C) +0.0 -1.2 N.D. -0.3 -0.1
carbon source propionate (C) +0.0 -0.3 N.D. -0.6 -0.6
carbon source L-Phenylalanine (C) -0.3 -0.6 N.D. -0.2 -0.3
carbon source propionate (C) +0.1 +0.0 N.D. -0.5 -0.9
stress Nickel (II) chloride 0.625 mM +0.1 +0.1 N.D. -0.8 -0.6
stress Polymyxin B sulfate 4 mg/ml -0.2 -0.2 N.D. -0.4 -0.3
carbon source L-Alanine (C) -0.0 -0.1 N.D. -0.7 -0.2
carbon source Ethanol (C) -0.1 -0.4 N.D. +0.1 -0.6
pH Minimal media pH9 -0.1 -0.5 N.D. -0.7 +0.4
motility outer cut, LB soft agar motility assay -0.1 +0.2 N.D. -0.3 -0.6
carbon source L-Citrulline +0.1 -0.9 N.D. -0.2 +0.2
carbon source acetate (C) -0.2 -0.2 N.D. -0.6 +0.2
stress Lomefloxacin 0.001 mM -0.2 -0.1 N.D. -0.9 +0.4
carbon source m-Inositol (C) +0.0 -1.0 N.D. +0.2 +0.1
carbon source D-Glucose +0.2 -0.4 N.D. -0.5 +0.1
stress copper (II) chloride 2.5 mM +0.3 -0.1 N.D. -0.5 -0.1
stress Doxycycline hyclate 0.0006 mg/ml +0.2 +0.3 N.D. -0.9 -0.0
carbon source Ethanol (C) +0.4 +0.2 N.D. -0.6 -0.3
stress Dimethyl Sulfoxide 7.5 vol% -0.1 +0.4 N.D. -0.4 -0.3
pH Minimal media pH9 +0.3 +0.2 N.D. -0.4 -0.3
carbon source acetate (C) -0.3 +0.1 N.D. -0.3 +0.4
stress methylglyoxal 0.009 vol% +0.3 +0.3 N.D. -0.2 -0.2
stress methylglyoxal 0.008 vol% +0.3 +0.2 N.D. -0.2 +0.3
nitrogen source Glycine (N) -0.1 +0.5 N.D. +0.3 -0.1
stress Sisomicin 0.008 mg/ml +0.5 -0.1 N.D. -0.2 +0.8
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