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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_22720 and AO353_22725 are separated by 30 nucleotides
AO353_22725 and AO353_22730 overlap by 4 nucleotides
AO353_22730 and AO353_22735 are separated by 208 nucleotides
AO353_22735 and AO353_22740 are separated by 77 nucleotides
AO353_22720: AO353_22720 - curli production assembly protein CsgG, at 4,976,597 to 4,977,277
_22720
AO353_22725: AO353_22725 - hypothetical protein, at 4,977,308 to 4,977,679
_22725
AO353_22730: AO353_22730 - hypothetical protein, at 4,977,676 to 4,978,335
_22730
AO353_22735: AO353_22735 - hypothetical protein, at 4,978,544 to 4,978,783
_22735
AO353_22740: AO353_22740 - sterol desaturase, at 4,978,861 to 4,980,015
_22740
Group
Condition
AO353
_22720
AO353
_22725
AO353
_22730
AO353
_22735
AO353
_22740
carbon source
D-Alanine (C)
-0.4
-0.2
+0.1
-1.7
+0.1
stress
sodium fluoride 6.25 mM
-0.5
-0.3
+0.0
-0.6
-0.5
stress
Cisplatin 0.006 mg/ml
-0.4
-0.2
-0.3
-1.4
+0.3
nitrogen source
Parabanic Acid (N)
+0.1
-0.6
+0.0
-0.7
-0.3
stress
copper (II) chloride 2.5 mM
-0.1
-0.6
+0.1
+0.1
-0.8
carbon source
L-Isoleucine (C)
+0.2
-0.1
-0.3
-0.2
-0.9
carbon source
L-Tryptophan (C)
+0.2
-0.5
-0.6
+0.4
-0.8
motility
outer cut, LB soft agar motility assay
-0.3
+0.2
+0.4
-1.5
-0.1
anaerobic
Anaerobic, acetate donor; nitrate and iron
+0.3
-0.6
+0.1
-0.2
-0.6
carbon source
m-Inositol (C)
-0.7
+0.4
-0.2
+0.1
-0.6
carbon source
pyruvate (C)
-0.3
-0.4
+0.1
+0.4
-0.6
motility
outer cut, LB soft agar motility assay
-0.3
+0.2
+0.4
-0.2
-1.0
carbon source
L-Citrulline
+0.2
-1.0
-0.1
+0.2
-0.2
stress
Furfuryl Alcohol 0.125 vol%
+0.4
+0.1
-0.1
-1.0
-0.1
stress
Vanillin 1.25 mM
+0.4
-0.2
+0.0
-0.9
+0.1
carbon source
L-Citrulline
+0.3
-0.4
-0.5
-0.4
+0.5
stress
Gentamicin 0.006 mg/ml
+0.3
+0.2
+0.1
-1.2
+0.2
stress
1-ethyl-3-methylimidazolium acetate 30 mM
+0.3
+0.4
-0.1
-0.8
-0.2
stress
Paraquat dichloride 0.032 mg/mL
-0.3
-0.4
-0.3
+1.0
-0.2
pH
Minimal media pH9
+0.7
-0.6
+0.3
+0.2
-0.4
stress
Doxycycline hyclate 0.0006 mg/ml
+0.1
-0.6
-0.2
+1.1
+0.1
carbon source
acetate (C)
+0.5
-0.6
-0.4
+1.0
+0.5
radiation
Ionizing radiation, 126.196 Gy,
+0.4
-0.0
-0.3
+1.3
-0.4
motility
outer cut, LB soft agar motility assay
-0.8
+0.9
+0.3
+0.6
+0.0
carbon source
L-Ornithine
-0.3
-0.4
+0.5
+0.8
+0.4
carbon source
L-Ornithine
+0.5
+0.3
-0.3
+0.7
-0.0
stress
Fusidic 0.125 mg/ml
-0.2
+0.2
+0.3
+0.6
+0.4
carbon source
L-Ornithine
+0.5
+0.3
+0.2
+0.3
+0.3
carbon source
L-Citrulline
+0.3
+0.3
-0.2
+0.8
+0.5
survival
4C survival; 672 hrs
+0.6
+0.4
-0.1
+1.1
+0.1
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