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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_22165 and AO353_22170 are separated by 241 nucleotides
AO353_22170 and AO353_22175 are separated by 375 nucleotides
AO353_22175 and AO353_22180 are separated by 416 nucleotides
AO353_22180 and AO353_22185 are separated by 28 nucleotides
AO353_22165: AO353_22165 - amino acid amidase, at 4,854,399 to 4,854,749
_22165
AO353_22170: AO353_22170 - 7-cyano-7-deazaguanine synthase, at 4,854,991 to 4,855,689
_22170
AO353_22175: AO353_22175 - oxidoreductase, at 4,856,065 to 4,857,447
_22175
AO353_22180: AO353_22180 - 2-oxobutyrate oxidase, at 4,857,864 to 4,858,901
_22180
AO353_22185: AO353_22185 - metal ABC transporter substrate-binding protein, at 4,858,930 to 4,859,745
_22185
Group
Condition
AO353
_22165
AO353
_22170
AO353
_22175
AO353
_22180
AO353
_22185
anaerobic
Anaerobic, acetate donor; nitrate and iron
N.D.
-0.5
+0.1
-1.1
+0.1
motility
outer cut, LB soft agar motility assay
N.D.
-1.0
-0.0
-0.0
-0.3
stress
Furfuryl Alcohol 0.125 vol%
N.D.
-0.1
-0.1
-0.7
-0.3
stress
Cisplatin 0.006 mg/ml
N.D.
-0.5
-0.1
-0.4
-0.1
nitrogen source
Adenosine (N)
N.D.
-0.0
-0.3
-0.5
-0.1
stress
Cisplatin 0.006 mg/ml
N.D.
+0.1
-0.1
-0.9
+0.1
carbon source
L-Phenylalanine (C)
N.D.
-0.9
+0.0
+0.0
+0.1
stress
Doxycycline hyclate 0.0006 mg/ml
N.D.
+0.4
-0.2
-1.0
+0.2
carbon source
L-Citrulline
N.D.
-0.0
+0.3
-0.5
-0.2
nitrogen source
nitrite (N)
N.D.
+0.3
+0.1
-0.8
-0.0
carbon source
Uridine (C)
N.D.
+0.3
-0.0
-0.6
-0.0
carbon source
propionate (C)
N.D.
-0.5
+0.1
+0.3
-0.1
stress
Thallium(I) acetate 0.016 mg/ml
N.D.
+0.1
-0.2
-0.3
+0.3
lb
LB
N.D.
+0.2
-0.1
-0.4
+0.2
carbon source
D-Glucose
N.D.
+0.5
-0.1
-0.4
+0.0
stress
Vancomycin 1 mg/ml
N.D.
+0.3
+0.2
-0.3
-0.2
carbon source
L-Ornithine
N.D.
-0.4
+0.2
-0.1
+0.3
carbon source
acetate (C)
N.D.
-0.3
+0.4
+0.3
-0.1
carbon source
L-Citrulline
N.D.
-0.5
+0.2
+0.2
+0.3
carbon source
L-Citrulline
N.D.
-0.4
+0.3
+0.1
+0.2
carbon source
Carnitine Hydrochloride (C)
N.D.
+0.4
+0.1
-0.3
+0.2
motility
outer cut, LB soft agar motility assay
N.D.
+0.7
+0.1
+0.2
-0.3
stress
Benzalkonium Chloride 0.012 mg/ml
N.D.
+0.2
+0.2
+0.4
-0.1
carbon source
acetate (C)
N.D.
+0.3
+0.2
+0.4
-0.1
carbon source
L-Leucine (C)
N.D.
+0.7
-0.1
+0.1
+0.1
anaerobic
N1B4wt with N2E3m denitrifying replicate C
N.D.
+0.5
+0.1
+0.1
+0.3
survival
4C survival; 672 hrs
N.D.
+0.2
+0.3
+0.4
+0.2
stress
Paraquat dichloride 0.032 mg/mL
N.D.
+0.2
+0.3
+0.7
+0.1
stress
sodium fluoride 6.25 mM
N.D.
+0.3
+0.2
+0.7
+0.2
carbon source
L-Ornithine
N.D.
-0.1
+0.3
+0.8
+0.5
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