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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_21825 and AO353_21830 are separated by 192 nucleotides
AO353_21830 and AO353_21835 are separated by 2 nucleotides
AO353_21835 and AO353_21840 are separated by 33 nucleotides
AO353_21840 and AO353_21845 are separated by 63 nucleotides
AO353_21825: AO353_21825 - GNAT family acetyltransferase, at 4,787,297 to 4,787,731
_21825
AO353_21830: AO353_21830 - chemotaxis protein CheY, at 4,787,924 to 4,788,508
_21830
AO353_21835: AO353_21835 - excinuclease ABC subunit C, at 4,788,511 to 4,790,334
_21835
AO353_21840: AO353_21840 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase, at 4,790,368 to 4,790,928
_21840
AO353_21845: AO353_21845 - tRNA-Gly, at 4,790,992 to 4,791,067
_21845
Group
Condition
AO353
_21825
AO353
_21830
AO353
_21835
AO353
_21840
AO353
_21845
stress
Cisplatin 0.006 mg/ml
+0.3
-2.5
-2.0
N.D.
N.D.
stress
Cisplatin 0.006 mg/ml
+0.2
-2.1
-2.3
N.D.
N.D.
stress
methylglyoxal 0.009 vol%
-0.3
-2.6
-0.9
N.D.
N.D.
carbon source
L-Citrulline
+0.5
+2.4
-0.6
N.D.
N.D.
anaerobic
Anaerobic, acetate donor; nitrate and iron
-0.4
+2.7
+0.4
N.D.
N.D.
carbon source
L-Ornithine
+0.8
+2.4
-0.2
N.D.
N.D.
denitrifying
Nitrate reduction, replicate C
+0.2
+3.1
-0.1
N.D.
N.D.
carbon source
L-Asparagine (C)
+0.4
+2.9
-0.1
N.D.
N.D.
motility
outer cut, LB soft agar motility assay
+1.0
+3.0
-0.7
N.D.
N.D.
stress
perchlorate 75 mM
+0.4
+3.3
-0.3
N.D.
N.D.
motility
outer cut, LB soft agar motility assay
+0.8
+3.2
-0.4
N.D.
N.D.
carbon source
L-Asparagine (C)
+0.5
+3.1
+0.0
N.D.
N.D.
carbon source
D-Lactate (C)
+0.4
+3.2
+0.0
N.D.
N.D.
carbon source
succinate (C)
+0.4
+3.6
-0.2
N.D.
N.D.
carbon source
succinate (C)
+0.2
+3.6
+0.1
N.D.
N.D.
carbon source
D-Glucose
+0.8
+3.2
-0.1
N.D.
N.D.
carbon source
L-Citrulline
+1.2
+2.8
-0.2
N.D.
N.D.
denitrifying
Nitrate reduction, replicate B
+0.4
+3.3
+0.2
N.D.
N.D.
carbon source
D-Glucose
+1.2
+3.1
-0.2
N.D.
N.D.
carbon source
Carnitine Hydrochloride (C)
+0.0
+4.1
+0.0
N.D.
N.D.
survival
4C survival; 360 hrs
+0.1
+4.2
-0.2
N.D.
N.D.
carbon source
propionate (C)
-0.2
+4.5
-0.2
N.D.
N.D.
carbon source
Putrescine (C)
+0.4
+3.9
+0.0
N.D.
N.D.
motility
outer cut, LB soft agar motility assay
+1.0
+3.6
-0.4
N.D.
N.D.
stress
Sisomicin 0.008 mg/ml
+0.5
+3.4
+0.6
N.D.
N.D.
nitrogen source
Adenosine (N)
+0.4
+4.6
-0.2
N.D.
N.D.
carbon source
acetate (C)
+0.3
+5.9
+0.1
N.D.
N.D.
carbon source
L-Serine (C)
+0.2
+6.0
+0.2
N.D.
N.D.
carbon source
acetate (C)
+1.1
+5.7
+0.1
N.D.
N.D.
survival
4C survival; 672 hrs
+1.1
+6.7
-0.6
N.D.
N.D.
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