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Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_21730 and AO353_21735 are separated by 111 nucleotides
AO353_21735 and AO353_21740 are separated by 37 nucleotides
AO353_21740 and AO353_21745 are separated by 129 nucleotides
AO353_21745 and AO353_21750 are separated by 24 nucleotides
AO353_21730: AO353_21730 - reactive intermediate/imine deaminase, at 4,763,546 to 4,763,935
_21730
AO353_21735: AO353_21735 - IclR family transcriptional regulator, at 4,764,047 to 4,764,811
_21735
AO353_21740: AO353_21740 - D-glucosaminate dehydratase (EC 4.3.1.9)
(from data)
, at 4,764,849 to 4,766,066
_21740
AO353_21745: AO353_21745 - ketodeoxygluconokinase, at 4,766,196 to 4,767,131
_21745
AO353_21750: AO353_21750 - peptidylprolyl isomerase, at 4,767,156 to 4,767,431
_21750
Group
Condition
AO353
_21730
AO353
_21735
AO353
_21740
AO353
_21745
AO353
_21750
nitrogen source
D-Glucosamine Hydrochloride (N)
N.D.
+0.7
-4.7
-0.1
+0.1
nitrogen source
Gly-DL-Asp (N)
N.D.
-2.1
-0.1
-0.0
-0.6
carbon source
D-Glucosamine Hydrochloride (C)
N.D.
-0.1
-2.4
-0.1
+0.1
motility
outer cut, LB soft agar motility assay
N.D.
-1.4
-0.4
+0.1
-0.6
survival
4C survival; 672 hrs
N.D.
-1.0
+0.2
-0.1
-1.0
anaerobic
Anaerobic, acetate donor; nitrate and iron
N.D.
-0.1
+0.3
-0.2
-1.6
stress
Doxycycline hyclate 0.0006 mg/ml
N.D.
-0.4
+0.6
-0.5
-1.3
carbon source
L-Glutamine (C)
N.D.
-0.1
-0.4
-0.3
-0.6
temperature
LB 10C
N.D.
-0.3
-0.4
+0.0
-0.7
carbon source
Glycerol (C)
N.D.
-0.3
-0.0
+0.1
-1.1
carbon source
D-Glucose
N.D.
-0.0
+0.1
-0.2
-1.1
carbon source
L-Citrulline
N.D.
-0.5
-0.2
+0.0
-0.5
stress
nitrite 10 mM
N.D.
-0.6
-0.2
+0.1
-0.4
stress
methylglyoxal 0.009 vol%
N.D.
-1.3
+0.1
+0.0
+0.1
stress
Rifampicin 0.005 mg/ml
N.D.
+0.1
-0.1
-0.3
-0.9
stress
acetate 68 mM
N.D.
+0.1
-0.0
-0.2
-0.9
stress
Tetracycline 0.0016 mg/ml
N.D.
+0.1
-0.0
+0.0
-1.1
stress
Nalidixic 0.004 mg/ml
N.D.
+0.2
+0.1
+0.1
-1.4
nitrogen source
Gly-Glu (N)
N.D.
-0.8
-0.1
-0.1
+0.2
carbon source
L-Tryptophan (C)
N.D.
+0.3
+0.5
+0.0
-1.4
stress
Cisplatin 0.006 mg/ml
N.D.
+0.5
+0.0
-0.3
-0.8
carbon source
L-Leucine (C)
N.D.
-0.7
+0.3
+0.2
-0.3
carbon source
L-Aspartic Acid (C)
N.D.
-0.8
-0.0
-0.1
+0.5
stress
Lomefloxacin 0.001 mM
N.D.
-0.3
-0.5
-0.5
+1.1
carbon source
L-Phenylalanine (C)
N.D.
-0.2
+0.0
-0.4
+0.9
carbon source
D-Alanine (C)
N.D.
-0.3
-0.1
-0.2
+0.9
pH
Minimal media pH9
N.D.
-0.6
-0.3
+0.1
+1.2
carbon source
L-Ornithine
N.D.
+0.0
+0.2
-0.3
+0.7
carbon source
m-Inositol (C)
N.D.
-0.0
+0.3
+0.2
+0.8
motility
outer cut, LB soft agar motility assay
N.D.
-0.3
+0.2
+0.1
+2.2
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