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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_21710 and AO353_21715 are separated by 52 nucleotides
AO353_21715 and AO353_21720 are separated by 10 nucleotides
AO353_21720 and AO353_21725 overlap by 4 nucleotides
AO353_21725 and AO353_21730 are separated by 29 nucleotides
AO353_21710: AO353_21710 - ABC transporter for D-glucosamine, periplasmic substrate-binding component
(from data)
, at 4,760,486 to 4,761,334
_21710
AO353_21715: AO353_21715 - ABC transporter for D-glucosamine, permease component 1
(from data)
, at 4,761,387 to 4,762,049
_21715
AO353_21720: AO353_21720 - ABC transporter for D-glucosamine, permease component 2
(from data)
, at 4,762,060 to 4,762,722
_21720
AO353_21725: AO353_21725 - ABC transporter for D-glucosamine, ATPase component
(from data)
, at 4,762,719 to 4,763,516
_21725
AO353_21730: AO353_21730 - reactive intermediate/imine deaminase, at 4,763,546 to 4,763,935
_21730
Group
Condition
AO353
_21710
AO353
_21715
AO353
_21720
AO353
_21725
AO353
_21730
nitrogen source
D-Glucosamine Hydrochloride (N)
-3.9
-3.8
-4.1
N.D.
N.D.
carbon source
D-Glucosamine Hydrochloride (C)
-2.1
-2.2
-1.7
N.D.
N.D.
motility
outer cut, LB soft agar motility assay
-0.6
+0.3
-0.6
N.D.
N.D.
stress
Thallium(I) acetate 0.008 mg/ml
-0.2
+0.0
-0.5
N.D.
N.D.
carbon source
acetate (C)
-0.2
-0.2
-0.3
N.D.
N.D.
stress
Cisplatin 0.006 mg/ml
-0.3
+0.0
-0.4
N.D.
N.D.
motility
outer cut, LB soft agar motility assay
-0.5
-0.3
+0.2
N.D.
N.D.
nitrogen source
Parabanic Acid (N)
-0.1
-0.3
-0.2
N.D.
N.D.
carbon source
acetate (C)
-0.2
-0.4
+0.1
N.D.
N.D.
pH
Minimal media pH7
-0.4
+0.1
-0.2
N.D.
N.D.
carbon source
L-Valine (C)
+0.1
-0.6
-0.0
N.D.
N.D.
nitrogen source
L-Lysine (N)
-0.2
-0.4
+0.1
N.D.
N.D.
lb
LB
-0.2
+0.1
-0.3
N.D.
N.D.
stress
Thallium(I) acetate 0.008 mg/ml
-0.2
+0.2
-0.4
N.D.
N.D.
carbon source
L-Serine (C)
+0.1
-0.3
-0.2
N.D.
N.D.
stress
Cobalt chloride 0.32 mM
-0.4
-0.0
+0.2
N.D.
N.D.
stress
Fusidic 0.125 mg/ml
-0.2
+0.2
-0.2
N.D.
N.D.
carbon source
D-Alanine (C)
+0.2
-0.5
+0.1
N.D.
N.D.
stress
Cholin acetate 19 mM
+0.2
+0.1
-0.4
N.D.
N.D.
carbon source
L-Phenylalanine (C)
+0.2
+0.1
-0.3
N.D.
N.D.
stress
Vancomycin 1 mg/ml
-0.1
+0.3
-0.3
N.D.
N.D.
carbon source
D-Serine (C)
-0.3
+0.3
+0.2
N.D.
N.D.
stress
Tetracycline 0.0016 mg/ml
+0.3
-0.3
+0.2
N.D.
N.D.
carbon source
D-Glucose
-0.3
+0.1
+0.4
N.D.
N.D.
stress
Furfuryl Alcohol 0.0625 vol%
+0.1
+0.4
-0.1
N.D.
N.D.
motility
outer cut, LB soft agar motility assay
+0.4
-0.1
+0.3
N.D.
N.D.
carbon source
L-Proline (C)
+0.2
+0.2
+0.2
N.D.
N.D.
denitrifying
Nitrate reduction, replicate B
+0.0
+0.4
+0.1
N.D.
N.D.
stress
Lomefloxacin 0.001 mM
+0.2
+0.2
+0.3
N.D.
N.D.
stress
Furfuryl Alcohol 0.25 vol%
+0.1
+0.5
+0.2
N.D.
N.D.
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