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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_21555 and AO353_21560 are separated by 226 nucleotides
AO353_21560 and AO353_21565 are separated by 62 nucleotides
AO353_21565 and AO353_21570 are separated by 18 nucleotides
AO353_21570 and AO353_21575 overlap by 4 nucleotides
AO353_21555: AO353_21555 - type VI secretion protein, at 4,718,531 to 4,719,103
_21555
AO353_21560: AO353_21560 - type VI secretion protein, at 4,719,330 to 4,719,779
_21560
AO353_21565: AO353_21565 - type VI secretion protein, at 4,719,842 to 4,721,173
_21565
AO353_21570: AO353_21570 - type VI secretion system protein ImpK, at 4,721,192 to 4,721,959
_21570
AO353_21575: AO353_21575 - type VI secretion system protein ImpL, at 4,721,956 to 4,725,774
_21575
Group
Condition
AO353
_21555
AO353
_21560
AO353
_21565
AO353
_21570
AO353
_21575
stress
Benzalkonium Chloride 0.012 mg/ml
+0.2
-1.1
+0.0
-0.4
-0.1
carbon source
L-Citrulline
-0.2
-0.7
-0.2
-0.1
-0.1
motility
outer cut, LB soft agar motility assay
+0.0
+0.2
-0.1
-1.2
-0.0
carbon source
L-Citrulline
-0.2
-0.7
+0.0
+0.2
-0.3
carbon source
L-Aspartic Acid (C)
-0.6
+0.2
-0.4
+0.0
-0.1
stress
Cisplatin 0.006 mg/ml
-0.5
-0.1
+0.2
-0.1
-0.3
motility
outer cut, LB soft agar motility assay
+0.5
-1.0
+0.1
-0.3
-0.2
survival
4C survival; 672 hrs
+0.3
-0.8
+0.1
-0.4
+0.1
carbon source
acetate (C)
+0.1
-0.4
-0.2
-0.6
+0.2
carbon source
Ethanol (C)
-0.1
+0.3
-0.3
-0.5
-0.1
stress
Thallium(I) acetate 0.016 mg/ml
-0.5
+0.2
+0.1
-0.3
-0.1
stress
1-ethyl-3-methylimidazolium acetate 20 mM
-0.5
-0.4
+0.3
+0.1
-0.1
pH
Minimal media pH7
-0.2
-0.9
+0.2
+0.3
+0.1
stress
1-ethyl-3-methylimidazolium acetate 15 mM
+0.2
-0.7
+0.2
-0.2
-0.0
carbon source
L-Leucine (C)
-0.1
-0.7
+0.2
+0.3
+0.1
carbon source
acetate (C)
+0.5
-0.2
-0.4
+0.1
-0.3
stress
Cobalt chloride 0.32 mM
+0.1
-0.5
-0.3
+0.5
-0.1
stress
Lomefloxacin 0.001 mM
-0.3
-0.2
-0.1
+0.7
-0.3
carbon source
L-Tryptophan (C)
-0.2
+0.3
-0.5
+0.2
-0.2
stress
Doxycycline hyclate 0.0002 mg/ml
-0.2
-0.6
+0.1
+0.5
+0.0
stress
Paraquat dichloride 0.032 mg/mL
-0.4
+0.6
+0.0
-0.2
-0.1
nitrogen source
Glycine (N)
+0.4
-0.4
-0.2
+0.2
-0.0
carbon source
L-Valine (C)
-0.1
+0.2
-0.2
+0.7
-0.3
lb
LB
-0.2
+0.4
+0.4
-0.3
+0.1
anaerobic
N1B4wt with N2E3m denitrifying replicate C
-0.2
+0.3
-0.3
+0.9
+0.1
stress
Cisplatin 0.006 mg/ml
-0.1
+0.7
+0.3
+0.1
-0.1
carbon source
L-Citrulline
+0.1
+0.5
+0.2
+0.2
-0.2
denitrifying
Nitrate reduction, replicate C
+0.3
-0.2
+0.5
+0.5
-0.1
stress
Doxycycline hyclate 0.0006 mg/ml
+0.7
-0.6
+0.1
+0.6
+0.2
anaerobic
Anaerobic, acetate donor; nitrate and iron
+0.1
+0.5
-0.5
+0.8
+0.3
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