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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_21125 and AO353_21130 are separated by 68 nucleotides
AO353_21130 and AO353_21135 are separated by 186 nucleotides
AO353_21135 and AO353_21140 are separated by 87 nucleotides
AO353_21140 and AO353_21145 are separated by 5 nucleotides
AO353_21125: AO353_21125 - lipopolysaccharide biosynthesis protein, at 4,618,496 to 4,620,046
_21125
AO353_21130: AO353_21130 - protein tyrosine kinase, at 4,620,115 to 4,620,864
_21130
AO353_21135: AO353_21135 - UDP-galactose-4-epimerase, at 4,621,051 to 4,622,028
_21135
AO353_21140: AO353_21140 - glycosyl hydrolase family 5, at 4,622,116 to 4,624,800
_21140
AO353_21145: AO353_21145 - ligase, at 4,624,806 to 4,626,332
_21145
Group
Condition
AO353
_21125
AO353
_21130
AO353
_21135
AO353
_21140
AO353
_21145
carbon source
D-Glucose
-0.1
-0.3
-0.1
-0.1
-0.1
carbon source
propionate (C)
-0.1
-0.2
-0.2
-0.0
+0.0
survival
4C survival; 672 hrs
+0.1
-0.5
-0.1
-0.0
-0.0
carbon source
L-Leucine (C)
-0.5
+0.0
-0.0
-0.1
+0.0
stress
Tetracycline 0.0016 mg/ml
+0.1
-0.4
-0.0
-0.1
-0.1
carbon source
L-Citrulline
-0.3
+0.1
-0.2
+0.0
-0.2
carbon source
L-Ornithine
-0.2
+0.1
-0.2
-0.0
-0.1
carbon source
L-Ornithine
-0.1
+0.2
-0.2
-0.1
-0.2
anaerobic
N1B4wt with N2E3m denitrifying replicate C
+0.1
-0.3
-0.0
-0.2
+0.0
carbon source
acetate (C)
+0.3
-0.3
-0.2
-0.0
-0.1
carbon source
D-Glucose
-0.5
+0.2
+0.0
+0.1
+0.0
motility
outer cut, LB soft agar motility assay
-0.0
-0.3
-0.1
+0.1
+0.1
stress
Thallium(I) acetate 0.016 mg/ml
+0.3
-0.2
-0.1
-0.0
-0.1
lb
LB
+0.2
-0.3
+0.0
+0.0
+0.0
stress
Doxycycline hyclate 0.0006 mg/ml
+0.3
-0.1
-0.2
-0.2
+0.2
motility
outer cut, LB soft agar motility assay
+0.1
-0.1
+0.3
-0.0
-0.3
stress
Doxycycline hyclate 0.0002 mg/ml
+0.3
-0.1
-0.1
-0.0
+0.1
stress
Lomefloxacin 0.001 mM
-0.1
+0.3
+0.3
-0.1
-0.1
denitrifying
Nitrate reduction, replicate B
-0.2
+0.5
+0.1
-0.1
-0.1
carbon source
L-Citrulline
-0.2
+0.4
+0.2
+0.1
-0.2
motility
outer cut, LB soft agar motility assay
-0.1
+0.2
+0.2
-0.0
+0.1
carbon source
L-Citrulline
-0.0
+0.2
+0.2
-0.1
+0.1
carbon source
L-Glutamine (C)
+0.0
-0.1
+0.2
+0.1
+0.2
nitrogen source
L-tyrosine (N)
-0.1
+0.2
-0.0
+0.2
+0.1
nitrogen source
Gly-DL-Asp (N)
-0.1
+0.3
+0.1
+0.1
+0.0
denitrifying
Nitrate reduction, replicate C
+0.3
-0.0
+0.2
-0.1
-0.1
stress
1-ethyl-3-methylimidazolium acetate 30 mM
+0.0
+0.3
-0.1
+0.0
+0.3
carbon source
D-Lactate (C)
+0.2
-0.1
+0.1
+0.1
+0.1
carbon source
Tween 20 (C)
+0.2
+0.2
-0.0
+0.1
+0.1
stress
copper (II) chloride 2.5 mM
+0.2
+0.2
-0.0
+0.1
+0.1
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