Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E3

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500 ntAO353_20905 and AO353_20910 overlap by 20 nucleotidesAO353_20910 and AO353_20915 are separated by 89 nucleotidesAO353_20915 and AO353_20925 are separated by 1518 nucleotidesAO353_20925 and AO353_20930 are separated by 446 nucleotides AO353_20905: AO353_20905 - integration host factor subunit alpha, at 4,574,256 to 4,574,558 _20905 AO353_20910: AO353_20910 - MerR family transcriptional regulator, at 4,574,539 to 4,574,895 _20910 AO353_20915: AO353_20915 - tRNA-Pro, at 4,574,985 to 4,575,061 _20915 AO353_20925: AO353_20925 - hypothetical protein, at 4,576,580 to 4,576,921 _20925 AO353_20930: AO353_20930 - hypothetical protein, at 4,577,368 to 4,577,622 _20930
Group Condition AO353_20905 AO353_20910 AO353_20915 AO353_20925 AO353_20930
stress Cisplatin 0.006 mg/ml N.D. -1.1 N.D. -2.3 -0.0
stress methylglyoxal 0.009 vol% N.D. -0.3 N.D. -2.1 -0.1
stress Cisplatin 0.006 mg/ml N.D. -1.5 N.D. -0.8 +0.2
carbon source propionate (C) N.D. -3.2 N.D. +1.6 +0.1
stress nitrate 200 mM N.D. -2.1 N.D. +1.4 -0.1
carbon source propionate (C) N.D. -2.7 N.D. +2.1 +0.0
stress perchlorate 75 mM N.D. -1.1 N.D. +1.8 +0.2
stress Rifampicin 0.005 mg/ml N.D. -0.4 N.D. +1.3 +0.2
survival 4C survival; 192 hrs N.D. -0.3 N.D. +1.6 -0.0
stress Sisomicin 0.008 mg/ml N.D. -0.8 N.D. +1.9 +0.3
carbon source L-Proline (C) N.D. -0.0 N.D. +1.7 -0.2
carbon source succinate (C) N.D. +0.5 N.D. +1.4 -0.2
carbon source L-Citrulline N.D. +1.0 N.D. +0.8 +0.2
carbon source D-Lactate (C) N.D. +0.5 N.D. +1.5 +0.0
motility outer cut, LB soft agar motility assay N.D. -0.0 N.D. +1.7 +0.3
carbon source Carnitine Hydrochloride (C) N.D. +0.5 N.D. +1.4 +0.1
carbon source L-Asparagine (C) N.D. +0.2 N.D. +1.7 +0.1
survival 4C survival; 360 hrs N.D. -0.3 N.D. +2.5 -0.1
carbon source succinate (C) N.D. +0.5 N.D. +1.7 -0.0
carbon source pyruvate (C) N.D. +0.6 N.D. +1.4 +0.1
anaerobic N1B4wt with N2E3m denitrifying replicate C N.D. +2.2 N.D. +0.2 +0.1
anaerobic Anaerobic, acetate donor; nitrate and iron N.D. +0.2 N.D. +1.9 +0.4
carbon source acetate (C) N.D. -0.6 N.D. +3.6 +0.0
survival 4C survival; 672 hrs N.D. -1.1 N.D. +3.9 +0.2
carbon source L-Citrulline N.D. +1.4 N.D. +1.7 -0.1
denitrifying Nitrate reduction, replicate B N.D. +1.0 N.D. +1.8 +0.2
carbon source L-Citrulline N.D. +1.5 N.D. +1.8 -0.1
carbon source acetate (C) N.D. -0.4 N.D. +3.6 +0.1
denitrifying Nitrate reduction, replicate C N.D. +1.2 N.D. +1.9 +0.3
carbon source L-Serine (C) N.D. +0.4 N.D. +3.4 +0.3
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