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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_20810 and AO353_20815 are separated by 235 nucleotides
AO353_20815 and AO353_20820 are separated by 57 nucleotides
AO353_20820 and AO353_20825 overlap by 4 nucleotides
AO353_20825 and AO353_20830 are separated by 3 nucleotides
AO353_20810: AO353_20810 - hypothetical protein, at 4,557,517 to 4,557,972
_20810
AO353_20815: AO353_20815 - LacI family transcriptional regulator, at 4,558,208 to 4,559,161
_20815
AO353_20820: AO353_20820 - sugar ABC transporter ATP-binding protein, at 4,559,219 to 4,560,772
_20820
AO353_20825: AO353_20825 - ABC transporter permease, at 4,560,769 to 4,561,746
_20825
AO353_20830: AO353_20830 - LacI family transcriptional regulator, at 4,561,750 to 4,562,769
_20830
Group
Condition
AO353
_20810
AO353
_20815
AO353
_20820
AO353
_20825
AO353
_20830
carbon source
D-Ribose (C)
-0.5
-1.9
-3.2
-3.1
-1.6
carbon source
D-Ribose (C)
+0.1
-2.2
-2.3
-3.0
-1.9
carbon source
Uridine (C)
+0.4
-1.4
-2.3
-3.3
-2.3
carbon source
L-Alanine (C)
-2.9
-0.7
+0.3
-0.4
-0.2
carbon source
acetate (C)
-1.7
-0.8
-0.3
-0.4
-0.5
carbon source
L-Citrulline
-0.5
-1.3
-0.1
-0.4
-0.2
stress
Fusidic 0.125 mg/ml
+0.4
-2.4
+0.1
-0.1
-0.2
carbon source
L-Ornithine
-1.0
-0.3
-0.0
-0.5
-0.3
anaerobic
N1B4wt with N2E3m denitrifying replicate C
-0.3
-1.4
-0.2
-0.1
-0.3
motility
outer cut, LB soft agar motility assay
-0.8
-0.4
-0.3
+0.1
-0.7
stress
Tetracycline 0.0016 mg/ml
-0.3
-1.2
-0.2
-0.1
-0.4
motility
outer cut, LB soft agar motility assay
-1.4
+0.5
-0.2
-0.2
-0.5
carbon source
L-Arginine (C)
-1.5
+0.3
+0.2
-0.3
-0.1
nitrogen source
L-Histidine (N)
+0.5
-1.3
-0.1
-0.1
-0.4
stress
Sisomicin 0.008 mg/ml
-2.3
+1.0
+0.0
+0.1
-0.1
stress
Cisplatin 0.006 mg/ml
-0.1
+0.4
-0.4
-0.5
-0.7
carbon source
acetate (C)
+0.4
+0.1
-0.2
-0.4
-0.9
carbon source
L-Serine (C)
-1.0
+0.7
+0.1
-0.1
-0.7
survival
4C survival; 672 hrs
+0.5
-0.9
+0.6
-0.0
-0.9
anaerobic
Anaerobic, acetate donor; nitrate and iron
-0.2
-1.3
+0.0
+0.4
+0.2
carbon source
L-Leucine (C)
-0.9
+0.5
-0.2
+0.1
-0.3
carbon source
L-Citrulline
+1.7
-2.1
-0.0
-0.3
-0.1
stress
copper (II) chloride 2.5 mM
-1.5
+0.8
+0.1
-0.1
+0.1
carbon source
L-Citrulline
+0.8
-0.4
-0.1
-0.8
+0.1
carbon source
propionate (C)
-0.5
+0.5
+0.5
-0.2
-0.6
carbon source
L-Ornithine
+0.6
+0.6
+0.1
-0.4
-0.3
carbon source
L-Citrulline
+1.4
+0.5
-0.1
-0.1
+0.2
nitrogen source
Gly-Glu (N)
+0.2
-1.4
-0.4
+2.3
+3.7
nitrogen source
Gly-DL-Asp (N)
-1.0
-0.5
-0.5
+3.0
+5.0
nitrogen source
Adenosine (N)
+0.1
+2.7
+3.7
+1.0
-0.6
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